Specific metabolic pathways of steroid sulfates in human liver microsomes

Kurt Einarsson; Jan Åke Gustafsson; Thomas Ihre; Magnus Ingelman-Sundberg

doi:10.1210/jcem-43-1-56

Specific metabolic pathways of steroid sulfates in human liver microsomes

Kurt Einarsson, Jan Åke Gustafsson, Thomas Ihre, Magnus Ingelman-Sundberg

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Abstract

The hydroxylation of steroid sulfates has been studied in liver microsomal preparations from adult humans. Ten different C₁₈, C₁₉ and C₂₁ steroid sulfates and the corresponding unconjugated steroids were used as substrates. In several cases it was found that steroid sulfates were efficiently hydroxylated in a way that differed both qualitatively and quantitatively from the hydroxylation of the corresponding unconjugated substrates. Only the hydrophobic (nonsulfurylated) end of the steroid sulfate molecule was hydroxylated. The steroids sulfurylated in position 3 were generally better substrates for the liver microsomal hydroxylase system than those sulfurylated in position 17. The findings that unconjugated and sulfoconjugated steroids are metabolized along different pathways in the liver may be of general significance. Sulfoconjugation and subsequent hydroxylation may also be an important pathway in the metabolism of xenobiotics in man.

Original language	English (US)
Pages (from-to)	56-63
Number of pages	8
Journal	Journal of Clinical Endocrinology and Metabolism
Volume	43
Issue number	1
DOIs	https://doi.org/10.1210/jcem-43-1-56
State	Published - 1976

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Biochemistry
Endocrinology
Clinical Biochemistry
Biochemistry, medical

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title = "Specific metabolic pathways of steroid sulfates in human liver microsomes",

abstract = "The hydroxylation of steroid sulfates has been studied in liver microsomal preparations from adult humans. Ten different C18, C19 and C21 steroid sulfates and the corresponding unconjugated steroids were used as substrates. In several cases it was found that steroid sulfates were efficiently hydroxylated in a way that differed both qualitatively and quantitatively from the hydroxylation of the corresponding unconjugated substrates. Only the hydrophobic (nonsulfurylated) end of the steroid sulfate molecule was hydroxylated. The steroids sulfurylated in position 3 were generally better substrates for the liver microsomal hydroxylase system than those sulfurylated in position 17. The findings that unconjugated and sulfoconjugated steroids are metabolized along different pathways in the liver may be of general significance. Sulfoconjugation and subsequent hydroxylation may also be an important pathway in the metabolism of xenobiotics in man.",

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T1 - Specific metabolic pathways of steroid sulfates in human liver microsomes

AU - Einarsson, Kurt

AU - Gustafsson, Jan Åke

AU - Ihre, Thomas

AU - Ingelman-Sundberg, Magnus

PY - 1976

Y1 - 1976

N2 - The hydroxylation of steroid sulfates has been studied in liver microsomal preparations from adult humans. Ten different C18, C19 and C21 steroid sulfates and the corresponding unconjugated steroids were used as substrates. In several cases it was found that steroid sulfates were efficiently hydroxylated in a way that differed both qualitatively and quantitatively from the hydroxylation of the corresponding unconjugated substrates. Only the hydrophobic (nonsulfurylated) end of the steroid sulfate molecule was hydroxylated. The steroids sulfurylated in position 3 were generally better substrates for the liver microsomal hydroxylase system than those sulfurylated in position 17. The findings that unconjugated and sulfoconjugated steroids are metabolized along different pathways in the liver may be of general significance. Sulfoconjugation and subsequent hydroxylation may also be an important pathway in the metabolism of xenobiotics in man.

AB - The hydroxylation of steroid sulfates has been studied in liver microsomal preparations from adult humans. Ten different C18, C19 and C21 steroid sulfates and the corresponding unconjugated steroids were used as substrates. In several cases it was found that steroid sulfates were efficiently hydroxylated in a way that differed both qualitatively and quantitatively from the hydroxylation of the corresponding unconjugated substrates. Only the hydrophobic (nonsulfurylated) end of the steroid sulfate molecule was hydroxylated. The steroids sulfurylated in position 3 were generally better substrates for the liver microsomal hydroxylase system than those sulfurylated in position 17. The findings that unconjugated and sulfoconjugated steroids are metabolized along different pathways in the liver may be of general significance. Sulfoconjugation and subsequent hydroxylation may also be an important pathway in the metabolism of xenobiotics in man.

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Specific metabolic pathways of steroid sulfates in human liver microsomes

Abstract

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