Spatial expression of a DNA repair gene, N-methylpurine-DNA glycosylase (MPG) during development in mice

Keun Kim Nam Keun Kim, Hwan Lee Shook Hwan Lee, Jong Sohn Tae Jong Sohn, R. Roy, S. Mitra, Min Chung Hyung Min Chung, Jae Ko Jung Jae Ko, Yul Cha Kwang Yul Cha

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Background: DNA repair is a crucial phenomenon that maintains the chromosome integrity of genome which are continuously damaged by endogenous and exogenous alkylating agents. If the damaged DNA is not repaired, it may lead to mutation, chromosomal aberration, aging and cancer. N-methylpurine-DNA glycosylase (MPG), a ubiquitous DNA repair enzyme, removes N-methylpurine and other damaged purines in DNA. Materials and Methods: MPG mRNA expression was revealed at various stages of mouse development from day 7.5 p.c. (post coitum) embryo to day 400 mature adult by Northern blot hybridization or RT-PCR. Results: MPG transcripts were abundant in the mouse embryo during pregnancy and in adult testis and ovary. The MPG mRNA level in the testis was low in 1-week-old mice, but the level showed its maximum among the organs tested in 4-week-old young adults. In placenta, the level of MPG mRNA continuously decreased from day 7.5 p.c. to day 17.5 p.c. Conclusions: The spatial expression of MPG gene is highly regulated. Transcription of MPG is maximum in rapidly dividing and growing tissues during development. These data suggest that an elevated rate of MPG transcription is required for DNA replication.

Original languageEnglish (US)
Pages (from-to)3037-3043
Number of pages7
JournalAnticancer Research
Volume20
Issue number5
StatePublished - Nov 9 2000

Keywords

  • Development
  • DNA repair
  • Embryo
  • Gene expression
  • MPG
  • Placenta

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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