TY - JOUR
T1 - Sorting by diffusion
T2 - An asymmetric obstacle course for continuous molecular separation
AU - Chou, Chia Fu
AU - Bakajin, Olgica
AU - Turner, Stephen W.P.
AU - Duke, Thomas A.J.
AU - Chan, Shirley S.
AU - Cox, Edward C.
AU - Craighead, Harold G.
AU - Austin, Robert H.
PY - 1999/11/23
Y1 - 1999/11/23
N2 - A separation technique employing a microfabricated sieve has been demonstrated by observing the motion of DNA molecules of different size. The sieve consists of a two-dimensional lattice of obstacles whose asymmetric disposition rectifies the Brownian motion of molecules driven through the device, causing them to follow paths that depend on their diffusion coefficient. A nominal 6% resolution by length of DNA molecules in the size range 15-30 kbp may be achieved in a 4-inch (10-cm) silicon wafer. The advantage of this method is that samples can be loaded and sorted continuously, in contrast to the batch mode commonly used in gel electrophoresis.
AB - A separation technique employing a microfabricated sieve has been demonstrated by observing the motion of DNA molecules of different size. The sieve consists of a two-dimensional lattice of obstacles whose asymmetric disposition rectifies the Brownian motion of molecules driven through the device, causing them to follow paths that depend on their diffusion coefficient. A nominal 6% resolution by length of DNA molecules in the size range 15-30 kbp may be achieved in a 4-inch (10-cm) silicon wafer. The advantage of this method is that samples can be loaded and sorted continuously, in contrast to the batch mode commonly used in gel electrophoresis.
KW - Brownian motion
KW - DNA fractionation
KW - Microfabricated array
UR - http://www.scopus.com/inward/record.url?scp=0033598696&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033598696&partnerID=8YFLogxK
U2 - 10.1073/pnas.96.24.13762
DO - 10.1073/pnas.96.24.13762
M3 - Article
C2 - 10570146
AN - SCOPUS:0033598696
VL - 96
SP - 13762
EP - 13765
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 24
ER -