Somatostatin Release from Dissociated Cerebral Cortical Cell Cultures

Roxanne M. Landon, Richard J. Robbins

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Primary monolayer cultures of dispersed fetal cerebral cortical cells can be used to measure the release of the neuropeptide, somatostatin. Three to fice percent of cellular IRS is released basally into KRB in 10 min. Basal release is stable for at least 60 min and stimulated levels of release can be induced by introducing ionophores,11 neurotransmitters,10,24 or peptides.25 The peptide content of the incubation samples is readily measured by a well-characterized, sensitive RIA. Table II summarizes the major factors that must be taken into consideration when developing this system for measuring peptide release.

Original languageEnglish (US)
Pages (from-to)412-424
Number of pages13
JournalMethods in Enzymology
Volume124
Issue numberC
DOIs
StatePublished - Jan 1 1986

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Somatostatin Release from Dissociated Cerebral Cortical Cell Cultures'. Together they form a unique fingerprint.

Cite this