TY - JOUR
T1 - Somatostatin Release from Dissociated Cerebral Cortical Cell Cultures
AU - Landon, Roxanne M.
AU - Robbins, Richard J.
PY - 1986/1/1
Y1 - 1986/1/1
N2 - Primary monolayer cultures of dispersed fetal cerebral cortical cells can be used to measure the release of the neuropeptide, somatostatin. Three to fice percent of cellular IRS is released basally into KRB in 10 min. Basal release is stable for at least 60 min and stimulated levels of release can be induced by introducing ionophores,11 neurotransmitters,10,24 or peptides.25 The peptide content of the incubation samples is readily measured by a well-characterized, sensitive RIA. Table II summarizes the major factors that must be taken into consideration when developing this system for measuring peptide release.
AB - Primary monolayer cultures of dispersed fetal cerebral cortical cells can be used to measure the release of the neuropeptide, somatostatin. Three to fice percent of cellular IRS is released basally into KRB in 10 min. Basal release is stable for at least 60 min and stimulated levels of release can be induced by introducing ionophores,11 neurotransmitters,10,24 or peptides.25 The peptide content of the incubation samples is readily measured by a well-characterized, sensitive RIA. Table II summarizes the major factors that must be taken into consideration when developing this system for measuring peptide release.
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U2 - 10.1016/0076-6879(86)24032-X
DO - 10.1016/0076-6879(86)24032-X
M3 - Article
C2 - 2872573
AN - SCOPUS:0022558752
SN - 0076-6879
VL - 124
SP - 412
EP - 424
JO - Methods in Enzymology
JF - Methods in Enzymology
IS - C
ER -