TY - JOUR
T1 - Somatostatin biosynthesis by cerebral cortical cells in monolayer culture
AU - Robbins, Richard J.
AU - Reichlin, Seymour
PY - 1983/8
Y1 - 1983/8
N2 - We examined the ability of isolated rat cerebral cortical cells to synthesize and secrete immunoreactive somatostatin (IRS). Dispersed telencephalic cells (107) from rat embryos contain approximately 24 pg IRS. The IRS content of 107 cultured cells increased steadily to a level of 2800 pg by 20 days in vitro. The IRS content in the medium followed a parallel production curve and was, in general, 10% of the cellular content. The nature of this IRS was characterized by gel filtration. Bio-Gel P-10 chromatography resolved four discrete IRS peaks. The first peak had an apparent molecular weight of 11,500. The second peak, which comigrated with synthetic Somatostatin-28, had a molecular weight of 7,000. The third peak had a molecular weight of 1,800, and the fourth peak, which comigrated with Somatostatin-14, had a molecular weight less than 1,800. Incubation with [3H]phenylalanine (Phe) revealed that 3H was incorporated into each peak. Isolation of 3H-labeled peak 4 was carried out by double affinity column purification. Sequential automated Edman degradation of this material revealed [3H]Phe in the positions that Phe occupies in Somatostatin-14. These studies document the ability of isolated rat cerebral cortical tissue to synthesize several forms of IRS, establishing them as endogenous cortical peptides.
AB - We examined the ability of isolated rat cerebral cortical cells to synthesize and secrete immunoreactive somatostatin (IRS). Dispersed telencephalic cells (107) from rat embryos contain approximately 24 pg IRS. The IRS content of 107 cultured cells increased steadily to a level of 2800 pg by 20 days in vitro. The IRS content in the medium followed a parallel production curve and was, in general, 10% of the cellular content. The nature of this IRS was characterized by gel filtration. Bio-Gel P-10 chromatography resolved four discrete IRS peaks. The first peak had an apparent molecular weight of 11,500. The second peak, which comigrated with synthetic Somatostatin-28, had a molecular weight of 7,000. The third peak had a molecular weight of 1,800, and the fourth peak, which comigrated with Somatostatin-14, had a molecular weight less than 1,800. Incubation with [3H]phenylalanine (Phe) revealed that 3H was incorporated into each peak. Isolation of 3H-labeled peak 4 was carried out by double affinity column purification. Sequential automated Edman degradation of this material revealed [3H]Phe in the positions that Phe occupies in Somatostatin-14. These studies document the ability of isolated rat cerebral cortical tissue to synthesize several forms of IRS, establishing them as endogenous cortical peptides.
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U2 - 10.1210/endo-113-2-574
DO - 10.1210/endo-113-2-574
M3 - Article
C2 - 6135597
AN - SCOPUS:0020604385
VL - 113
SP - 574
EP - 581
JO - Endocrinology
JF - Endocrinology
SN - 0013-7227
IS - 2
ER -