Somatostatin as a mediator of the effect of neurotensin on pentagastrin-stimulated acid secretion in rats

Robert A. Hammer, Alejandro Ochoa, Cesar Fernandez, Atilla Ertan, Akira Arimura

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


Neurotensin and somatostatin have both been shown to inhibit gastric acid secretion, but no interaction between these peptides has been demonstrated. To determine whether somatostatin might be a mediator of neurotensin's effect on pentagastrin-stimulated gastric acid secretion, we performed the following three experiments. First, we collected 0.2-ml samples of portal venous blood as frequently as every 5 min, and we confirmed a significant release of somatostatin-like immunoreactivity into portal venous blood during neurotensin-induced inhibition of acid secretion. This release of somatostatin-like immunoreactivity and inhibition of acid secretion were only seen in pentobarbital-anesthetized rats, but no sustained release of somatostatin-like immunoreactivity or inhibition of acid secretion occurred in urethane-anesthetized animals. In the second experiment, we analyzed portal plasma by high pressure liquid chromatography, and found that portal somatostatin-like immunoreactivity in blood collected during neurotensin infusion was composed of a single peak corresponding to somatostatin-14. In the third experiment, we found that infusion of antibody to somatostatin prevented neurotensin from inhibiting pentagastrin-stimulated acid secretion. Taken together, these data show that somatostatin, possibly from the stomach itself, is a necessary mediator of neurotensin's inhibitory effect in pentobarbital-anesthetized rats.

Original languageEnglish (US)
Pages (from-to)1175-1179
Number of pages5
Issue number6
StatePublished - Jan 1 1992


  • Bioassay
  • Enterogastrone
  • Pentobarbital
  • Portal blood
  • Radioimmunoassay
  • Urethane

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Physiology
  • Endocrinology
  • Biochemistry


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