SNAP19 mediates the assembly of a functional core promoter complex (SNAP(c)) shared by RNA polymerases II and III

R. William Henry; Vivek Mittal; Beicong Ma; Ryuji Kobayashi; Nouria Hernandez

doi:10.1101/gad.12.17.2664

SNAP19 mediates the assembly of a functional core promoter complex (SNAP(c)) shared by RNA polymerases II and III

R. William Henry, Vivek Mittal, Beicong Ma, Ryuji Kobayashi, Nouria Hernandez

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72 Scopus citations

Abstract

The basal transcription factor SNAP(c) binds to the PSE, a core element in the RNA polymerase II and III human snRNA promoters. SNAP(c) contains at least four subunits, but it has not been possible to assemble a fully defined recombinant SNAP(c). Here we reconstitute SNAP(c) from five recombinant subunits, SNAP43, SNAP45, SNAP50, SNAP190, and a newly identified subunit, SNAP19. This recombinant complex binds specifically to the PSE and directs both RNA polymerase II and III snRNA gene transcription. Thus, the same core SNAP(c) nucleates the assembly of two classes of initiation complexes.

Original language	English (US)
Pages (from-to)	2664-2672
Number of pages	9
Journal	Genes and Development
Volume	12
Issue number	17
DOIs	https://doi.org/10.1101/gad.12.17.2664
State	Published - Sep 1 1998

Keywords

Human snRNA promoters
RNA polymerases
SNAP(c)
Transcription

ASJC Scopus subject areas

Genetics
Developmental Biology

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10.1101/gad.12.17.2664

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title = "SNAP19 mediates the assembly of a functional core promoter complex (SNAP(c)) shared by RNA polymerases II and III",

abstract = "The basal transcription factor SNAP(c) binds to the PSE, a core element in the RNA polymerase II and III human snRNA promoters. SNAP(c) contains at least four subunits, but it has not been possible to assemble a fully defined recombinant SNAP(c). Here we reconstitute SNAP(c) from five recombinant subunits, SNAP43, SNAP45, SNAP50, SNAP190, and a newly identified subunit, SNAP19. This recombinant complex binds specifically to the PSE and directs both RNA polymerase II and III snRNA gene transcription. Thus, the same core SNAP(c) nucleates the assembly of two classes of initiation complexes.",

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T1 - SNAP19 mediates the assembly of a functional core promoter complex (SNAP(c)) shared by RNA polymerases II and III

AU - Henry, R. William

AU - Mittal, Vivek

AU - Ma, Beicong

AU - Kobayashi, Ryuji

AU - Hernandez, Nouria

PY - 1998/9/1

Y1 - 1998/9/1

N2 - The basal transcription factor SNAP(c) binds to the PSE, a core element in the RNA polymerase II and III human snRNA promoters. SNAP(c) contains at least four subunits, but it has not been possible to assemble a fully defined recombinant SNAP(c). Here we reconstitute SNAP(c) from five recombinant subunits, SNAP43, SNAP45, SNAP50, SNAP190, and a newly identified subunit, SNAP19. This recombinant complex binds specifically to the PSE and directs both RNA polymerase II and III snRNA gene transcription. Thus, the same core SNAP(c) nucleates the assembly of two classes of initiation complexes.

AB - The basal transcription factor SNAP(c) binds to the PSE, a core element in the RNA polymerase II and III human snRNA promoters. SNAP(c) contains at least four subunits, but it has not been possible to assemble a fully defined recombinant SNAP(c). Here we reconstitute SNAP(c) from five recombinant subunits, SNAP43, SNAP45, SNAP50, SNAP190, and a newly identified subunit, SNAP19. This recombinant complex binds specifically to the PSE and directs both RNA polymerase II and III snRNA gene transcription. Thus, the same core SNAP(c) nucleates the assembly of two classes of initiation complexes.

KW - Human snRNA promoters

KW - RNA polymerases

KW - SNAP(c)

KW - Transcription

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SNAP19 mediates the assembly of a functional core promoter complex (SNAP(c)) shared by RNA polymerases II and III

Abstract

Keywords

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