TY - JOUR
T1 - SNAP19 mediates the assembly of a functional core promoter complex (SNAP(c)) shared by RNA polymerases II and III
AU - Henry, R. William
AU - Mittal, Vivek
AU - Ma, Beicong
AU - Kobayashi, Ryuji
AU - Hernandez, Nouria
PY - 1998/9/1
Y1 - 1998/9/1
N2 - The basal transcription factor SNAP(c) binds to the PSE, a core element in the RNA polymerase II and III human snRNA promoters. SNAP(c) contains at least four subunits, but it has not been possible to assemble a fully defined recombinant SNAP(c). Here we reconstitute SNAP(c) from five recombinant subunits, SNAP43, SNAP45, SNAP50, SNAP190, and a newly identified subunit, SNAP19. This recombinant complex binds specifically to the PSE and directs both RNA polymerase II and III snRNA gene transcription. Thus, the same core SNAP(c) nucleates the assembly of two classes of initiation complexes.
AB - The basal transcription factor SNAP(c) binds to the PSE, a core element in the RNA polymerase II and III human snRNA promoters. SNAP(c) contains at least four subunits, but it has not been possible to assemble a fully defined recombinant SNAP(c). Here we reconstitute SNAP(c) from five recombinant subunits, SNAP43, SNAP45, SNAP50, SNAP190, and a newly identified subunit, SNAP19. This recombinant complex binds specifically to the PSE and directs both RNA polymerase II and III snRNA gene transcription. Thus, the same core SNAP(c) nucleates the assembly of two classes of initiation complexes.
KW - Human snRNA promoters
KW - RNA polymerases
KW - SNAP(c)
KW - Transcription
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U2 - 10.1101/gad.12.17.2664
DO - 10.1101/gad.12.17.2664
M3 - Article
C2 - 9732265
AN - SCOPUS:0032167810
SN - 0890-9369
VL - 12
SP - 2664
EP - 2672
JO - Genes and Development
JF - Genes and Development
IS - 17
ER -