Small interfering RNA targeting the PINK1 induces apoptosis in dopaminergic cells SH-SY5Y

Hao Deng, Joseph Jankovic, Yi Guo, Wenjie Xie, Weidong Le

Research output: Contribution to journalArticle

122 Scopus citations

Abstract

PTEN-induced kinase 1 (PINK1) is a recently identified gene, mutations of which cause levodopa-responsive parkinsonism. An over-expression of wild-type PINK1 protects neurons from stress-induced mitochondrial dysfunction and apoptosis. We studied the effects of PINK1 suppression using small interfering RNA (siRNA), which can inhibit PINK1 mRNA expression up to 87%, and decrease PINK1 protein up to 80% in human dopaminergic cell line SH-SY5Y. Incubation with PINK1 siRNA decreased SH-SY5Y cell viability and significantly increased MPP+ or rotenone-induced cytotoxicity. Our results indicate that reduction in PINK1 expression can trigger apoptotic process that can be exacerbated by the presence of MPP+ or rotenone. These findings support the hypothesis that PINK1 participates in the protection of dopaminergic neurons.

Original languageEnglish (US)
Pages (from-to)1133-1138
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume337
Issue number4
DOIs
StatePublished - Dec 2 2005

Keywords

  • Apoptosis
  • Parkinsonism
  • PINK1
  • RNAi
  • SH-SY5Y cells

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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