TY - JOUR
T1 - Skeletal myogenic potential of human and mouse neural stem cells
AU - Galli, Rossella
AU - Borello, Ugo
AU - Gritti, Angela
AU - Minasi, M. Giulia
AU - Bjornson, Christopher
AU - Coletta, Marcello
AU - Mora, Marina
AU - Cusella De Angelis, M. Gabriella
AU - Fiocco, Roberta
AU - Cossu, Giulio
AU - Vescovi, Angelo L.
N1 - Funding Information:
This work was supported by the EEC (Grants QLRT000894, QLRT-1999-31471), the Italian Telethon Foundation, the Spinal Cord Society (MN) (A.L.V.) and the Fondazione Pasteur-Cenci Bolognetti (G.C.). We thank Don Ward for help with the English.
Copyright:
Copyright 2004 Elsevier Science B.V., Amsterdam. All rights reserved.
PY - 2000/10
Y1 - 2000/10
N2 - Distinct cell lineages established early in development are usually maintained throughout adulthood. Thus, adult stem cells have been thought to generate differentiated cells specific to the tissue in which they reside. This view has been challenged; for example, neural stem cells can generate cells that normally originate from a different germ layer. Here we show that acutely isolated and clonally derived neural stem cells from mice and humans could produce skeletal myotubes in vitro and in vivo, the latter following transplantation into adult animals. Myogenic conversion in vitro required direct exposure to myoblasts, and was blocked if neural cells were clustered. Thus, a community effect between neural cells may override such myogenic induction. We conclude that neural stem cells, which generate neurons, glia and blood cells, can also produce skeletal muscle cells, and can undergo various patterns of differentiation depending on exposure to appropriate epigenetic signals in mature tissues.
AB - Distinct cell lineages established early in development are usually maintained throughout adulthood. Thus, adult stem cells have been thought to generate differentiated cells specific to the tissue in which they reside. This view has been challenged; for example, neural stem cells can generate cells that normally originate from a different germ layer. Here we show that acutely isolated and clonally derived neural stem cells from mice and humans could produce skeletal myotubes in vitro and in vivo, the latter following transplantation into adult animals. Myogenic conversion in vitro required direct exposure to myoblasts, and was blocked if neural cells were clustered. Thus, a community effect between neural cells may override such myogenic induction. We conclude that neural stem cells, which generate neurons, glia and blood cells, can also produce skeletal muscle cells, and can undergo various patterns of differentiation depending on exposure to appropriate epigenetic signals in mature tissues.
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U2 - 10.1038/79924
DO - 10.1038/79924
M3 - Article
C2 - 11017170
AN - SCOPUS:0033804048
VL - 3
SP - 986
EP - 991
JO - Nature Neuroscience
JF - Nature Neuroscience
SN - 1097-6256
IS - 10
ER -