Abstract
The size-specific capturing mechanism for Mycobacterium tuberculosis (MTB) cells is presented using a microtip-sensor. When a microtip is withdrawn from a solution drop, enriched cells by an alternating current (AC) electric field are selectively captured onto the tip due to capillary- and viscous forces. The size-specificity on the 50 μm-diameter tip is studied by using polystyrene microspheres. The size-specific capturing mechanism is applied to capture MTB cells from a sample mixture containing MTB and Drosophila S2 cells. Under the competition between capillary- and viscous forces, MTB cells are captured onto the microtip by the viscous force, while Drosophila S2 cells are removed from the tip by the capillary force. The microtip-sensor yields the detection limit of 800 cells/mL for MTB cells, which is one of the highest-sensitivity immunosensors.
Original language | English (US) |
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Title of host publication | ASME 2010 International Mechanical Engineering Congress and Exposition, IMECE 2010 |
Pages | 263-266 |
Number of pages | 4 |
Volume | 2 |
DOIs | |
State | Published - Dec 1 2010 |
Event | ASME 2010 International Mechanical Engineering Congress and Exposition, IMECE 2010 - Vancouver, BC, Canada Duration: Nov 12 2010 → Nov 18 2010 |
Other
Other | ASME 2010 International Mechanical Engineering Congress and Exposition, IMECE 2010 |
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Country/Territory | Canada |
City | Vancouver, BC |
Period | 11/12/10 → 11/18/10 |
ASJC Scopus subject areas
- Mechanical Engineering