Size-specific immunofluorescence of Mycobacterium tuberculosis cells by using a microtip-sensor

Woon Hong Yeo; Fong Li Chou; Dayong Gao; Amy Q. Shen; Jae Hyun Chung; Kyong Hoon Lee

doi:10.1115/IMECE2010-37719

Size-specific immunofluorescence of Mycobacterium tuberculosis cells by using a microtip-sensor

Woon Hong Yeo, Fong Li Chou, Dayong Gao, Amy Q. Shen, Jae Hyun Chung, Kyong Hoon Lee

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The size-specific capturing mechanism for Mycobacterium tuberculosis (MTB) cells is presented using a microtip-sensor. When a microtip is withdrawn from a solution drop, enriched cells by an alternating current (AC) electric field are selectively captured onto the tip due to capillary- and viscous forces. The size-specificity on the 50 μm-diameter tip is studied by using polystyrene microspheres. The size-specific capturing mechanism is applied to capture MTB cells from a sample mixture containing MTB and Drosophila S2 cells. Under the competition between capillary- and viscous forces, MTB cells are captured onto the microtip by the viscous force, while Drosophila S2 cells are removed from the tip by the capillary force. The microtip-sensor yields the detection limit of 800 cells/mL for MTB cells, which is one of the highest-sensitivity immunosensors.

Original language	English (US)
Title of host publication	ASME 2010 International Mechanical Engineering Congress and Exposition, IMECE 2010
Pages	263-266
Number of pages	4
Volume	2
DOIs	https://doi.org/10.1115/IMECE2010-37719
State	Published - Dec 1 2010
Event	ASME 2010 International Mechanical Engineering Congress and Exposition, IMECE 2010 - Vancouver, BC, Canada Duration: Nov 12 2010 → Nov 18 2010

Other

Other	ASME 2010 International Mechanical Engineering Congress and Exposition, IMECE 2010
Country/Territory	Canada
City	Vancouver, BC
Period	11/12/10 → 11/18/10

ASJC Scopus subject areas

Mechanical Engineering

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10.1115/IMECE2010-37719

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Yeo, WH, Chou, FL, Gao, D, Shen, AQ, Chung, JH & Lee, KH 2010, Size-specific immunofluorescence of Mycobacterium tuberculosis cells by using a microtip-sensor. in ASME 2010 International Mechanical Engineering Congress and Exposition, IMECE 2010. vol. 2, pp. 263-266, ASME 2010 International Mechanical Engineering Congress and Exposition, IMECE 2010, Vancouver, BC, Canada, 11/12/10. https://doi.org/10.1115/IMECE2010-37719

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abstract = "The size-specific capturing mechanism for Mycobacterium tuberculosis (MTB) cells is presented using a microtip-sensor. When a microtip is withdrawn from a solution drop, enriched cells by an alternating current (AC) electric field are selectively captured onto the tip due to capillary- and viscous forces. The size-specificity on the 50 μm-diameter tip is studied by using polystyrene microspheres. The size-specific capturing mechanism is applied to capture MTB cells from a sample mixture containing MTB and Drosophila S2 cells. Under the competition between capillary- and viscous forces, MTB cells are captured onto the microtip by the viscous force, while Drosophila S2 cells are removed from the tip by the capillary force. The microtip-sensor yields the detection limit of 800 cells/mL for MTB cells, which is one of the highest-sensitivity immunosensors.",

author = "Yeo, {Woon Hong} and Chou, {Fong Li} and Dayong Gao and Shen, {Amy Q.} and Chung, {Jae Hyun} and Lee, {Kyong Hoon}",

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AU - Yeo, Woon Hong

AU - Chou, Fong Li

AU - Gao, Dayong

AU - Shen, Amy Q.

AU - Chung, Jae Hyun

AU - Lee, Kyong Hoon

PY - 2010/12/1

Y1 - 2010/12/1

N2 - The size-specific capturing mechanism for Mycobacterium tuberculosis (MTB) cells is presented using a microtip-sensor. When a microtip is withdrawn from a solution drop, enriched cells by an alternating current (AC) electric field are selectively captured onto the tip due to capillary- and viscous forces. The size-specificity on the 50 μm-diameter tip is studied by using polystyrene microspheres. The size-specific capturing mechanism is applied to capture MTB cells from a sample mixture containing MTB and Drosophila S2 cells. Under the competition between capillary- and viscous forces, MTB cells are captured onto the microtip by the viscous force, while Drosophila S2 cells are removed from the tip by the capillary force. The microtip-sensor yields the detection limit of 800 cells/mL for MTB cells, which is one of the highest-sensitivity immunosensors.

AB - The size-specific capturing mechanism for Mycobacterium tuberculosis (MTB) cells is presented using a microtip-sensor. When a microtip is withdrawn from a solution drop, enriched cells by an alternating current (AC) electric field are selectively captured onto the tip due to capillary- and viscous forces. The size-specificity on the 50 μm-diameter tip is studied by using polystyrene microspheres. The size-specific capturing mechanism is applied to capture MTB cells from a sample mixture containing MTB and Drosophila S2 cells. Under the competition between capillary- and viscous forces, MTB cells are captured onto the microtip by the viscous force, while Drosophila S2 cells are removed from the tip by the capillary force. The microtip-sensor yields the detection limit of 800 cells/mL for MTB cells, which is one of the highest-sensitivity immunosensors.

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ER -

Size-specific immunofluorescence of Mycobacterium tuberculosis cells by using a microtip-sensor

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