Size-specific immunofluorescence of Mycobacterium tuberculosis cells by using a microtip-sensor

Woon Hong Yeo, Fong Li Chou, Dayong Gao, Amy Q. Shen, Jae Hyun Chung, Kyong Hoon Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The size-specific capturing mechanism for Mycobacterium tuberculosis (MTB) cells is presented using a microtip-sensor. When a microtip is withdrawn from a solution drop, enriched cells by an alternating current (AC) electric field are selectively captured onto the tip due to capillary- and viscous forces. The size-specificity on the 50 μm-diameter tip is studied by using polystyrene microspheres. The size-specific capturing mechanism is applied to capture MTB cells from a sample mixture containing MTB and Drosophila S2 cells. Under the competition between capillary- and viscous forces, MTB cells are captured onto the microtip by the viscous force, while Drosophila S2 cells are removed from the tip by the capillary force. The microtip-sensor yields the detection limit of 800 cells/mL for MTB cells, which is one of the highest-sensitivity immunosensors.

Original languageEnglish (US)
Title of host publicationASME 2010 International Mechanical Engineering Congress and Exposition, IMECE 2010
Pages263-266
Number of pages4
Volume2
DOIs
StatePublished - Dec 1 2010
EventASME 2010 International Mechanical Engineering Congress and Exposition, IMECE 2010 - Vancouver, BC, Canada
Duration: Nov 12 2010Nov 18 2010

Other

OtherASME 2010 International Mechanical Engineering Congress and Exposition, IMECE 2010
CountryCanada
CityVancouver, BC
Period11/12/1011/18/10

ASJC Scopus subject areas

  • Mechanical Engineering

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