Six mRNAs with different 5′ ends are encoded by a single γ-glutamyltransferase gene in mouse

S. Rajagopalan; D. F. Wan; G. M. Habib; A. R. Sepulveda; M. R. McLeod; R. M. Lebovitz; Michael W. Lieberman

Six mRNAs with different 5′ ends are encoded by a single γ-glutamyltransferase gene in mouse

S. Rajagopalan, D. F. Wan, G. M. Habib, A. R. Sepulveda, M. R. McLeod, R. M. Lebovitz, Michael W. Lieberman

Research output: Contribution to journal › Article › peer-review

Abstract

The 5′ region of the mouse γ-glutamyltransferase (γGT; EC 2.3.2.2) gene has been cloned and analyzed. This analysis, combined with sequence information obtained from γGT cDNA clones, indicates that in mouse a single γGT gene codes for six different mRNAs that differ in their 5′ sequences. Analysis of steady-state levels of γGT RNA reveals different expression patterns for these RNAs in different organs. The six different 5′ sequences are widely separated within a 10-kb region and three of them show 75-86% identity with the three known rat γGT cDNAs. Although the heterogeneity of the 5′ ends of γGT RNAs may be explained in part by alternative splicing, it is likely that multiple promoters are involved in their generation.

Original language	English (US)
Pages (from-to)	6179-6183
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	90
Issue number	13
State	Published - Jul 1 1993

Keywords

Gene expression
Kidney
Promoter
Transcription

ASJC Scopus subject areas

General
Genetics

Cite this

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title = "Six mRNAs with different 5′ ends are encoded by a single γ-glutamyltransferase gene in mouse",

abstract = "The 5′ region of the mouse γ-glutamyltransferase (γGT; EC 2.3.2.2) gene has been cloned and analyzed. This analysis, combined with sequence information obtained from γGT cDNA clones, indicates that in mouse a single γGT gene codes for six different mRNAs that differ in their 5′ sequences. Analysis of steady-state levels of γGT RNA reveals different expression patterns for these RNAs in different organs. The six different 5′ sequences are widely separated within a 10-kb region and three of them show 75-86% identity with the three known rat γGT cDNAs. Although the heterogeneity of the 5′ ends of γGT RNAs may be explained in part by alternative splicing, it is likely that multiple promoters are involved in their generation.",

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T1 - Six mRNAs with different 5′ ends are encoded by a single γ-glutamyltransferase gene in mouse

AU - Rajagopalan, S.

AU - Wan, D. F.

AU - Habib, G. M.

AU - Sepulveda, A. R.

AU - McLeod, M. R.

AU - Lebovitz, R. M.

AU - Lieberman, Michael W.

PY - 1993/7/1

Y1 - 1993/7/1

N2 - The 5′ region of the mouse γ-glutamyltransferase (γGT; EC 2.3.2.2) gene has been cloned and analyzed. This analysis, combined with sequence information obtained from γGT cDNA clones, indicates that in mouse a single γGT gene codes for six different mRNAs that differ in their 5′ sequences. Analysis of steady-state levels of γGT RNA reveals different expression patterns for these RNAs in different organs. The six different 5′ sequences are widely separated within a 10-kb region and three of them show 75-86% identity with the three known rat γGT cDNAs. Although the heterogeneity of the 5′ ends of γGT RNAs may be explained in part by alternative splicing, it is likely that multiple promoters are involved in their generation.

AB - The 5′ region of the mouse γ-glutamyltransferase (γGT; EC 2.3.2.2) gene has been cloned and analyzed. This analysis, combined with sequence information obtained from γGT cDNA clones, indicates that in mouse a single γGT gene codes for six different mRNAs that differ in their 5′ sequences. Analysis of steady-state levels of γGT RNA reveals different expression patterns for these RNAs in different organs. The six different 5′ sequences are widely separated within a 10-kb region and three of them show 75-86% identity with the three known rat γGT cDNAs. Although the heterogeneity of the 5′ ends of γGT RNAs may be explained in part by alternative splicing, it is likely that multiple promoters are involved in their generation.

KW - Gene expression

KW - Kidney

KW - Promoter

KW - Transcription

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Six mRNAs with different 5′ ends are encoded by a single γ-glutamyltransferase gene in mouse

Abstract

Keywords

ASJC Scopus subject areas

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