Single-step analysis of individual conjugated bile acids in human bile using1H NMR spectroscopy

G. A.Nagana Gowda, Omkar B. Ijare, B. S. Somashekar, Ajay Sharma, V. K. Kapoor, C. L. Khetrapal

Research output: Contribution to journalArticlepeer-review

45 Scopus citations


1H and 13C NMR spectra of intact human bile were assigned using one-dimensional (1H and 13C) and two-dimensional (1H-1H and 1H-13C) experiments. Individual conjugated bile acids-glycocholic acid, glycodeoxycholic acid, glycochenodeoxycholic acid, taurocholic acid, taurodeoxycholic acid, and taurochenodeoxycholic acid-were identified. The bile acids were quantified accurately and individually in a single step by using distinct and characteristic amide signals. Making use of 13C NMR, the study also suggests a way to analyze unconjugated bile acids separately, if present. Chemical shift assignments and rapid single-step analysis of individual conjugated bile acids from intact bile presented herein may have immense utility in the study of bile acid metabolism and deeper understanding of hepatobiliary diseases.

Original languageEnglish (US)
Pages (from-to)591-603
Number of pages13
Issue number6
StatePublished - Jun 2006

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology


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