TY - JOUR
T1 - Single prolonged stress decreases glutamate, glutamine, and creatine concentrations in the rat medial prefrontal cortex
AU - Knox, Dayan
AU - Perrine, Shane A.
AU - George, Sophie A.
AU - Galloway, Matthew P.
AU - Liberzon, Israel
N1 - Funding Information:
The research in this manuscript was supported by a Veteran Affairs Merit award and Department of Defense grant W81XWH-08-1-0661 to Dr. Israel Liberzon, NIDA award DA024760 to Dr. Shane Perrine, DA R01-16736 to Dr. Matthew Galloway, the Joe Young Fund for Psychiatry Research, and the Fund for Medical Research in Anesthesiology at Wayne State University School of Medicine. We would like to thank Dr. Farhad Ghoddoussi for his expert technical assistance.
PY - 2010/8
Y1 - 2010/8
N2 - Application of single prolonged stress (SPS) in rats induces changes in neuroendocrine function and arousal that are characteristic of post traumatic stress disorder (PTSD). PTSD, in humans, is associated with decreased neural activity in the prefrontal cortex, increased neural activity in the amygdala complex, and reduced neuronal integrity in the hippocampus. However, the extent to which SPS models these aspects of PTSD has not been established. In order to address this, we used high-resolution magic angle spinning proton magnetic resonance spectroscopy (HR-MAS 1H MRS) ex vivo to assay levels of neurochemicals critical for energy metabolism (creatine and lactate), excitatory (glutamate and glutamine) and inhibitory (gamma amino butyric acid (GABA)) neurotransmission, and neuronal integrity (N-acetylaspartate (NAA)) in the medial prefrontal cortex (mPFC), amygdala complex, and hippocampus of SPS and control rats. Glutamate, glutamine, and creatine levels were decreased in the mPFC of SPS rats when compared to controls, which suggests decreased excitatory tone in this region. SPS did not alter the neurochemical profiles of either the hippocampus or amygdala. These data suggest that SPS selectively attenuates excitatory tone, without a disruption of neuronal integrity, in the mPFC.
AB - Application of single prolonged stress (SPS) in rats induces changes in neuroendocrine function and arousal that are characteristic of post traumatic stress disorder (PTSD). PTSD, in humans, is associated with decreased neural activity in the prefrontal cortex, increased neural activity in the amygdala complex, and reduced neuronal integrity in the hippocampus. However, the extent to which SPS models these aspects of PTSD has not been established. In order to address this, we used high-resolution magic angle spinning proton magnetic resonance spectroscopy (HR-MAS 1H MRS) ex vivo to assay levels of neurochemicals critical for energy metabolism (creatine and lactate), excitatory (glutamate and glutamine) and inhibitory (gamma amino butyric acid (GABA)) neurotransmission, and neuronal integrity (N-acetylaspartate (NAA)) in the medial prefrontal cortex (mPFC), amygdala complex, and hippocampus of SPS and control rats. Glutamate, glutamine, and creatine levels were decreased in the mPFC of SPS rats when compared to controls, which suggests decreased excitatory tone in this region. SPS did not alter the neurochemical profiles of either the hippocampus or amygdala. These data suggest that SPS selectively attenuates excitatory tone, without a disruption of neuronal integrity, in the mPFC.
KW - Anxiety
KW - Emotional regulation
KW - GABA
KW - Glutamate
KW - PTSD
KW - Proton magnetic resonance spectroscopy
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U2 - 10.1016/j.neulet.2010.05.052
DO - 10.1016/j.neulet.2010.05.052
M3 - Article
C2 - 20546834
AN - SCOPUS:77954383813
SN - 0304-3940
VL - 480
SP - 16
EP - 20
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 1
ER -