Zero-Mode Waveguides were first introduced for Fluorescence Correlation Spectroscopy at micromolar dye concentrations. We show that combining zero-mode waveguides with fluorescence correlation spectroscopy in a continuous flow mixer avoids the compression of the FCS signal due to fluid transport at channel velocities up to ∼ 17 mm/s. We derive an analytic scaling relationship |δkON/kON| = |δkOFF/k OFF| ∼ kON+kOFF/kON 0.1D B/DF-DB e/√SNR converting this flow velocity insensitivity to improved kinetic rate certainty in time-resolved mixing experiments. Thus zero-mode waveguides make FCS suitable for direct kinetics measurements in rapid continuous flow.
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics