Single molecule analysis of electrodynamically stretched DNA in nanochannels

Samuel M. Stavis, Christian H. Reccius, John T. Mannion, Larry P. Walker, Harold G. Craighead

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A rapid (∼20 ms) and precise (∼110 nm) analytical method is presented for stretching and interrogating single DNA molecules. DNA strands were driven electrophoretically from a nanoslit into a nanochannel to confine and dynamically elongate them for repeated detection via laser induced fluorescence. Photon bursts were modelled analytically and DNA conformation, length, speed, and fluorescence intensity were measured simultaneously. The confluence of these measurements is a step forward towards applications limited by analysis time and spatial resolution, such as single molecule genomic sequencing. Several aspects of the translocation of DNA through a nanochannel were investigated, including stretching and speed as a function of electric field, and speed as a function of folding and length.

Original languageEnglish
Title of host publication12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
PublisherChemical and Biological Microsystems Society
Pages1510-1512
Number of pages3
StatePublished - Jan 1 2008
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: Oct 12 2008Oct 16 2008

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period10/12/0810/16/08

Keywords

  • DNA
  • Nanochannel
  • Nanofluidic
  • Single molecule

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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