Single molecule analysis of electrodynamically stretched DNA in nanochannels

Samuel M. Stavis; Christian H. Reccius; John T. Mannion; Larry P. Walker; Harold G. Craighead

Single molecule analysis of electrodynamically stretched DNA in nanochannels

Samuel M. Stavis, Christian H. Reccius, John T. Mannion, Larry P. Walker, Harold G. Craighead

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A rapid (∼20 ms) and precise (∼110 nm) analytical method is presented for stretching and interrogating single DNA molecules. DNA strands were driven electrophoretically from a nanoslit into a nanochannel to confine and dynamically elongate them for repeated detection via laser induced fluorescence. Photon bursts were modelled analytically and DNA conformation, length, speed, and fluorescence intensity were measured simultaneously. The confluence of these measurements is a step forward towards applications limited by analysis time and spatial resolution, such as single molecule genomic sequencing. Several aspects of the translocation of DNA through a nanochannel were investigated, including stretching and speed as a function of electric field, and speed as a function of folding and length.

Original language	English
Title of host publication	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
Publisher	Chemical and Biological Microsystems Society
Pages	1510-1512
Number of pages	3
State	Published - Jan 1 2008
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: Oct 12 2008 → Oct 16 2008

Other

Other	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	10/12/08 → 10/16/08

Keywords

DNA
Nanochannel
Nanofluidic
Single molecule

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

Single molecule analysis of electrodynamically stretched DNA in nanochannels. / Stavis, Samuel M.; Reccius, Christian H.; Mannion, John T. et al.

12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, 2008. p. 1510-1512.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Stavis, SM, Reccius, CH, Mannion, JT, Walker, LP & Craighead, HG 2008, Single molecule analysis of electrodynamically stretched DNA in nanochannels. in 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, pp. 1510-1512, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States, 10/12/08.

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title = "Single molecule analysis of electrodynamically stretched DNA in nanochannels",

abstract = "A rapid (∼20 ms) and precise (∼110 nm) analytical method is presented for stretching and interrogating single DNA molecules. DNA strands were driven electrophoretically from a nanoslit into a nanochannel to confine and dynamically elongate them for repeated detection via laser induced fluorescence. Photon bursts were modelled analytically and DNA conformation, length, speed, and fluorescence intensity were measured simultaneously. The confluence of these measurements is a step forward towards applications limited by analysis time and spatial resolution, such as single molecule genomic sequencing. Several aspects of the translocation of DNA through a nanochannel were investigated, including stretching and speed as a function of electric field, and speed as a function of folding and length.",

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T1 - Single molecule analysis of electrodynamically stretched DNA in nanochannels

AU - Stavis, Samuel M.

AU - Reccius, Christian H.

AU - Mannion, John T.

AU - Walker, Larry P.

AU - Craighead, Harold G.

PY - 2008/1/1

Y1 - 2008/1/1

N2 - A rapid (∼20 ms) and precise (∼110 nm) analytical method is presented for stretching and interrogating single DNA molecules. DNA strands were driven electrophoretically from a nanoslit into a nanochannel to confine and dynamically elongate them for repeated detection via laser induced fluorescence. Photon bursts were modelled analytically and DNA conformation, length, speed, and fluorescence intensity were measured simultaneously. The confluence of these measurements is a step forward towards applications limited by analysis time and spatial resolution, such as single molecule genomic sequencing. Several aspects of the translocation of DNA through a nanochannel were investigated, including stretching and speed as a function of electric field, and speed as a function of folding and length.

AB - A rapid (∼20 ms) and precise (∼110 nm) analytical method is presented for stretching and interrogating single DNA molecules. DNA strands were driven electrophoretically from a nanoslit into a nanochannel to confine and dynamically elongate them for repeated detection via laser induced fluorescence. Photon bursts were modelled analytically and DNA conformation, length, speed, and fluorescence intensity were measured simultaneously. The confluence of these measurements is a step forward towards applications limited by analysis time and spatial resolution, such as single molecule genomic sequencing. Several aspects of the translocation of DNA through a nanochannel were investigated, including stretching and speed as a function of electric field, and speed as a function of folding and length.

KW - DNA

KW - Nanochannel

KW - Nanofluidic

KW - Single molecule

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M3 - Conference contribution

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EP - 1512

BT - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference

PB - Chemical and Biological Microsystems Society

Y2 - 12 October 2008 through 16 October 2008

ER -

Single molecule analysis of electrodynamically stretched DNA in nanochannels

Abstract

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Keywords

ASJC Scopus subject areas

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