Single cell whole genome amplification via micropillar arrays for reducing amplification bias

Harvey C. Tian, Harold G. Craighead

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a valveless microfluidic device for single cell capture and on-chip whole genome amplification of immobilized DNA. This device uses a series of closely spaced micropillars to physically entrap cells and its genomic DNA (gDNA) upon cell lysis. Our past work has demonstrated high efficiency DNA capture using micropillar arrays [1]. Here, we demonstrate the ability to perform in-channel chemistries, such as DNA amplification on single cells. We report that out of six gene loci sampled, we detect all six loci from amplified product collected from our device compared to two out of six in control samples.

Original languageEnglish (US)
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages1231-1232
Number of pages2
ISBN (Electronic)9780692941836
StatePublished - Jan 1 2020
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: Oct 22 2017Oct 26 2017

Other

Other21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
CountryUnited States
CitySavannah
Period10/22/1710/26/17

Keywords

  • Amplification Bias
  • DNA
  • MDA
  • Microfluidics
  • Single Cell
  • Whole Genome Amplification

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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