Single cell nanoparticle tracking to model cell cycle dynamics and compartmental inheritance

Rachel J. Errington, Martyn R. Brown, Oscar F. Silvestre, Kerenza L. Njoh, Sally C. Chappell, Imtiaz A. Khan, Paul Rees, Steve P. Wilks, Paul J. Smith, Huw D. Summers

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Single cell encoding with quantum dots as live cell optical tracers for deriving proliferation parameters has been developed using modelling to investigate cell cycle and proliferative outputs of human osteosarcoma cells undergoing mitotic bypass and endocycle routing. A computer-based simulation of the evolving cell population provides information on the dilution and segregation of nanoparticle dose cell by cell division and allows quantitative assessment of patterns of division, at both single cell and including whole population level cell cycle routing, with no a priori knowledge of the population proliferation potential. the output therefore provides a unique mitotic distribution function that represents a convolution of cell cycle kinetics (cell division) and the partitioning coefficient for the labelled cell compartment (daughter-daughter inheritance or lineage asymmetry). The current study has shown that the cellular quantum dot fluorescence reduced over time as the particles were diluted by the process of cell division and had the properties of a non-random highly asymmetric event. Asymmetric nanoparticle segregation in the endosomal compartment has major implications on cell-fate determining signaling pathways and could lead to an understanding of the origins of unique proliferation and drug-resistance characteristics within a tumour cell lineage.

Original languageEnglish (US)
Pages (from-to)121-130
Number of pages10
JournalCell Cycle
Volume9
Issue number1
DOIs
StatePublished - Jan 1 2010

Keywords

  • Cell cycle
  • Cell lineage encoding
  • Cell proliferation
  • Flow cytometry
  • Nanocrystal quantum dots

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Developmental Biology

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