Single-cell electrical lysis of erythrocytes detects deficiencies in the cytoskeletal protein network

Ning Bao; Gayani C. Kodippili; Katie M. Giger; Velia M. Fowler; Philip S. Low; Chang Lu

doi:10.1039/c1lc20365g

Single-cell electrical lysis of erythrocytes detects deficiencies in the cytoskeletal protein network

Ning Bao, Gayani C. Kodippili, Katie M. Giger, Velia M. Fowler, Philip S. Low, Chang Lu

Houston Methodist

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

The network of erythrocyte cytoskeletal proteins significantly influences erythrocyte physical and biological properties. Here we show that the kinetics of erythrocyte lysis during exposure to an electric field is sensitively correlated with defects in the cytoskeletal network. Histograms compiled from single-cell electrical lysis data show characteristics of erythrocyte populations that are deficient in a specific cytoskeletal protein, revealing the presence of cell subpopulations.

Original language	English (US)
Pages (from-to)	3053-3056
Number of pages	4
Journal	Lab on a Chip
Volume	11
Issue number	18
DOIs	https://doi.org/10.1039/c1lc20365g
State	Published - Sep 21 2011

ASJC Scopus subject areas

Bioengineering
Biochemistry
General Chemistry
Biomedical Engineering

Access to Document

10.1039/c1lc20365g

Cite this

@article{a714c14c535e4a50a162289f3e10c462,

title = "Single-cell electrical lysis of erythrocytes detects deficiencies in the cytoskeletal protein network",

abstract = "The network of erythrocyte cytoskeletal proteins significantly influences erythrocyte physical and biological properties. Here we show that the kinetics of erythrocyte lysis during exposure to an electric field is sensitively correlated with defects in the cytoskeletal network. Histograms compiled from single-cell electrical lysis data show characteristics of erythrocyte populations that are deficient in a specific cytoskeletal protein, revealing the presence of cell subpopulations.",

author = "Ning Bao and Kodippili, {Gayani C.} and Giger, {Katie M.} and Fowler, {Velia M.} and Low, {Philip S.} and Chang Lu",

year = "2011",

month = sep,

day = "21",

doi = "10.1039/c1lc20365g",

language = "English (US)",

volume = "11",

pages = "3053--3056",

journal = "Lab on a Chip",

issn = "1473-0197",

publisher = "Royal Society of Chemistry",

number = "18",

}

TY - JOUR

T1 - Single-cell electrical lysis of erythrocytes detects deficiencies in the cytoskeletal protein network

AU - Bao, Ning

AU - Kodippili, Gayani C.

AU - Giger, Katie M.

AU - Fowler, Velia M.

AU - Low, Philip S.

AU - Lu, Chang

PY - 2011/9/21

Y1 - 2011/9/21

N2 - The network of erythrocyte cytoskeletal proteins significantly influences erythrocyte physical and biological properties. Here we show that the kinetics of erythrocyte lysis during exposure to an electric field is sensitively correlated with defects in the cytoskeletal network. Histograms compiled from single-cell electrical lysis data show characteristics of erythrocyte populations that are deficient in a specific cytoskeletal protein, revealing the presence of cell subpopulations.

AB - The network of erythrocyte cytoskeletal proteins significantly influences erythrocyte physical and biological properties. Here we show that the kinetics of erythrocyte lysis during exposure to an electric field is sensitively correlated with defects in the cytoskeletal network. Histograms compiled from single-cell electrical lysis data show characteristics of erythrocyte populations that are deficient in a specific cytoskeletal protein, revealing the presence of cell subpopulations.

UR - http://www.scopus.com/inward/record.url?scp=80052216518&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052216518&partnerID=8YFLogxK

U2 - 10.1039/c1lc20365g

DO - 10.1039/c1lc20365g

M3 - Article

C2 - 21785802

AN - SCOPUS:80052216518

SN - 1473-0197

VL - 11

SP - 3053

EP - 3056

JO - Lab on a Chip

JF - Lab on a Chip

IS - 18

ER -

Single-cell electrical lysis of erythrocytes detects deficiencies in the cytoskeletal protein network

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this