Simultaneous Quantification of Multiple Cancer Biomarkers in Blood Samples through DNA-Assisted Nanopore Sensing

Lei Liu, Ting Li, Shouwen Zhang, Peng Song, Bingyuan Guo, Yuliang Zhao, Hai Chen Wu

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Protein biomarkers in blood have been widely used in the early diagnosis of disease. However, simultaneous detection of many biomarkers in a single sample remains challenging. Herein, we show that the combination of a sandwich assay and DNA-assisted nanopore sensing could unambiguously identify and quantify several antigens in a mixture. We use five barcode DNAs to label different gold nanoparticles that can selectively bind specific antigens. After the completion of the sandwich assay, barcode DNAs are released and subject to nanopore translocation tests. The distinct current signatures generated by each barcode DNA allow simultaneous quantification of biomarkers at picomolar level in clinical samples. This approach would be very useful for accurate and multiplexed quantification of cancer-associated biomarkers within a very small sample volume, which is critical for non-invasive early diagnosis of cancer.

Original languageEnglish (US)
Pages (from-to)11882-11887
Number of pages6
JournalAngewandte Chemie - International Edition
Volume57
Issue number37
DOIs
StatePublished - Sep 10 2018

Keywords

  • antigens
  • barcode DNA
  • biomarkers
  • multiplexed detection
  • nanopore sensing

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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