Simulations of redox mediation within bioactive hydrogels of amperometric biosensors

Walter Torres, Anthony Guiseppi-Elie

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Highly hydrated bioactive hydrogels containing immobilized oxidoreductase enzymes and immobilized redox mediators were simulated as the biorecognition layer of amperometric biosensors. The linear dynamic range of the amperometric response of mediated biosensors increases and moves to higher concentration brackets with an increase in the concentration of mediator. This informs the design of biosensors that target the same analyte but possesses several independently addressable electrodes modified with hydrogels that contain different concentrations of mediator. Increases in enzyme concentration increase the linear dynamic range but does not alter the sensitivity of amperometric biosensors. Both sensitivity and linear dynamic range of mediated amperometric enzyme biosensors may be "tuned" by varying the concentrations of the enzyme and the mediator. Simulations effectively guide the initial domains of study of complex systems such as implantable biosensors.

Original languageEnglish (US)
Pages (from-to)1923-1928
Number of pages6
JournalJournal of Macromolecular Science, Part A: Pure and Applied Chemistry
Volume43
Issue number12
DOIs
StatePublished - Dec 1 2006

Keywords

  • Biosensors
  • Enzymes
  • Hydrogels
  • Mediation
  • Simulation

ASJC Scopus subject areas

  • Ceramics and Composites
  • Chemistry(all)
  • Polymers and Plastics
  • Materials Chemistry

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