Simple pulse-acquire NMR methods for the quantitative analysis of calcium, magnesium and sodium in human serum

B. S. Somashekar, Omkar B. Ijare, G. A. Nagana Gowda, V. Ramesh, Sushil Gupta, C. L. Khetrapal

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Simple pulse-acquire NMR methods are presented for accurate quantitation of calcium (Ca2+), magnesium (Mg2+) and sodium (Na+) in human serum. Ca2+ and Mg2+ can be determined simultaneously by 1H NMR via their EDTA (ethylenediaminetetraacetic acid) complexes. Spectra are acquired before and after addition of EDTA, and the difference spectrum is used for integration of the signals from the complexes relative to an internal reference. Serum sodium can also be determined through pulse-acquire 23Na NMR by integration of the free sodium signal relative to a reference signal (Na+ + EDTA in a coaxial capillary tube). The method shows excellent accuracy and precision for all three metal ions. Slow chemical exchange between complexed and free EDTA at the natural pH of serum does not limit the accuracy of the determination of Ca2+ and Mg2+, and the large errors associated with spin-echo spectral editing methods are avoided.

Original languageEnglish (US)
Pages (from-to)254-260
Number of pages7
JournalSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume65
Issue number2
DOIs
StatePublished - Oct 2006

Keywords

  • Calcium
  • Magnesium
  • NMR
  • Quantitation
  • Serum
  • Sodium

ASJC Scopus subject areas

  • Analytical Chemistry
  • Atomic and Molecular Physics, and Optics
  • Instrumentation
  • Spectroscopy

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