Silver-Nanoparticle-Embedded Porous Silicon Disks Enabled SERS Signal Amplification for Selective Glutathione Detection

Yang Bu; Guixian Zhu; Shengliang Li; Ruogu Qi; Gauri Bhave; Dechen Zhang; Ruixuan Han; Dali Sun; Xiangfeng Liu; Zhongbo Hu; Xuewu Liu

doi:10.1021/acsanm.7b00290

Silver-Nanoparticle-Embedded Porous Silicon Disks Enabled SERS Signal Amplification for Selective Glutathione Detection

Yang Bu, Guixian Zhu, Shengliang Li, Ruogu Qi, Gauri Bhave, Dechen Zhang, Ruixuan Han, Dali Sun, Xiangfeng Liu, Zhongbo Hu, Xuewu Liu

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

As the major redox couple and nonprotein thiol source in human tissues, the level of glutathione (GSH) has been a concern for its relation with many diseases. However, the similar physical and chemical properties of interference molecules such as cysteine (Cys) and homocysteine (Hcy) make discriminative detection of GSH in complex biological fluids challenging. Here we report a novel surface-enhanced Raman scattering (SERS) platform, based on silver-nanoparticle-embedded porous silicon disks (PSDs/Ag) substrates for highly sensitive and selective detection of GSH in biofluids. Silver nanoparticles (AgNPs) were reductively synthesized and aggregated directly into pores of PSDs, achieving a SERS enhancement factor (EF) up to 2.59 × 10⁷. Ellman's reagent 5,5′-ditho-bis (2-nitrobenzoic acid) (DTNB) was selected as the Raman reactive reporting agent, and the GSH quantification was determined using enzymatic recycling method, and allowed the detection limit of GSH to be down to 74.9 nM using a portable Raman spectrometer. Moreover, the significantly overwhelmed enhancement ratio of GSH over other substances enables the discrimination of GSH detection in complex biofluids.

Original language	English (US)
Pages (from-to)	410-417
Number of pages	8
Journal	ACS Applied Nano Materials
Volume	1
Issue number	1
DOIs	https://doi.org/10.1021/acsanm.7b00290
State	Published - Jan 1 2018

Keywords

SERS
biosensor
glutathione
porous silicon
signal amplification
silver nanoparticles

ASJC Scopus subject areas

Materials Science(all)

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10.1021/acsanm.7b00290

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title = "Silver-Nanoparticle-Embedded Porous Silicon Disks Enabled SERS Signal Amplification for Selective Glutathione Detection",

abstract = "As the major redox couple and nonprotein thiol source in human tissues, the level of glutathione (GSH) has been a concern for its relation with many diseases. However, the similar physical and chemical properties of interference molecules such as cysteine (Cys) and homocysteine (Hcy) make discriminative detection of GSH in complex biological fluids challenging. Here we report a novel surface-enhanced Raman scattering (SERS) platform, based on silver-nanoparticle-embedded porous silicon disks (PSDs/Ag) substrates for highly sensitive and selective detection of GSH in biofluids. Silver nanoparticles (AgNPs) were reductively synthesized and aggregated directly into pores of PSDs, achieving a SERS enhancement factor (EF) up to 2.59 × 107. Ellman's reagent 5,5′-ditho-bis (2-nitrobenzoic acid) (DTNB) was selected as the Raman reactive reporting agent, and the GSH quantification was determined using enzymatic recycling method, and allowed the detection limit of GSH to be down to 74.9 nM using a portable Raman spectrometer. Moreover, the significantly overwhelmed enhancement ratio of GSH over other substances enables the discrimination of GSH detection in complex biofluids.",

keywords = "SERS, biosensor, glutathione, porous silicon, signal amplification, silver nanoparticles",

author = "Yang Bu and Guixian Zhu and Shengliang Li and Ruogu Qi and Gauri Bhave and Dechen Zhang and Ruixuan Han and Dali Sun and Xiangfeng Liu and Zhongbo Hu and Xuewu Liu",

note = "Funding Information: The authors thank Dr. Jianhua Gu for his help in SEM imaging. Microfabrication of PSD was performed at the Microelectronics Research Center at the University of Texas at Austin. The authors acknowledge financial support from the following sources: Grants NIH 1R21CA190024-01, DOD W81XWH-12-1-0414, National Natural Science Foundation of China Grant 81571791, and the Houston Methodist Research Institute. Publisher Copyright: {\textcopyright} 2017 American Chemical Society.",

year = "2018",

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doi = "10.1021/acsanm.7b00290",

language = "English (US)",

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AU - Bu, Yang

AU - Zhu, Guixian

AU - Li, Shengliang

AU - Qi, Ruogu

AU - Bhave, Gauri

AU - Zhang, Dechen

AU - Han, Ruixuan

AU - Sun, Dali

AU - Liu, Xiangfeng

AU - Hu, Zhongbo

AU - Liu, Xuewu

N1 - Funding Information: The authors thank Dr. Jianhua Gu for his help in SEM imaging. Microfabrication of PSD was performed at the Microelectronics Research Center at the University of Texas at Austin. The authors acknowledge financial support from the following sources: Grants NIH 1R21CA190024-01, DOD W81XWH-12-1-0414, National Natural Science Foundation of China Grant 81571791, and the Houston Methodist Research Institute. Publisher Copyright: © 2017 American Chemical Society.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - As the major redox couple and nonprotein thiol source in human tissues, the level of glutathione (GSH) has been a concern for its relation with many diseases. However, the similar physical and chemical properties of interference molecules such as cysteine (Cys) and homocysteine (Hcy) make discriminative detection of GSH in complex biological fluids challenging. Here we report a novel surface-enhanced Raman scattering (SERS) platform, based on silver-nanoparticle-embedded porous silicon disks (PSDs/Ag) substrates for highly sensitive and selective detection of GSH in biofluids. Silver nanoparticles (AgNPs) were reductively synthesized and aggregated directly into pores of PSDs, achieving a SERS enhancement factor (EF) up to 2.59 × 107. Ellman's reagent 5,5′-ditho-bis (2-nitrobenzoic acid) (DTNB) was selected as the Raman reactive reporting agent, and the GSH quantification was determined using enzymatic recycling method, and allowed the detection limit of GSH to be down to 74.9 nM using a portable Raman spectrometer. Moreover, the significantly overwhelmed enhancement ratio of GSH over other substances enables the discrimination of GSH detection in complex biofluids.

AB - As the major redox couple and nonprotein thiol source in human tissues, the level of glutathione (GSH) has been a concern for its relation with many diseases. However, the similar physical and chemical properties of interference molecules such as cysteine (Cys) and homocysteine (Hcy) make discriminative detection of GSH in complex biological fluids challenging. Here we report a novel surface-enhanced Raman scattering (SERS) platform, based on silver-nanoparticle-embedded porous silicon disks (PSDs/Ag) substrates for highly sensitive and selective detection of GSH in biofluids. Silver nanoparticles (AgNPs) were reductively synthesized and aggregated directly into pores of PSDs, achieving a SERS enhancement factor (EF) up to 2.59 × 107. Ellman's reagent 5,5′-ditho-bis (2-nitrobenzoic acid) (DTNB) was selected as the Raman reactive reporting agent, and the GSH quantification was determined using enzymatic recycling method, and allowed the detection limit of GSH to be down to 74.9 nM using a portable Raman spectrometer. Moreover, the significantly overwhelmed enhancement ratio of GSH over other substances enables the discrimination of GSH detection in complex biofluids.

KW - SERS

KW - biosensor

KW - glutathione

KW - porous silicon

KW - signal amplification

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Silver-Nanoparticle-Embedded Porous Silicon Disks Enabled SERS Signal Amplification for Selective Glutathione Detection

Abstract

Keywords

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