Significantly improved oral uptake of amikacin in FVB mice in the presence of CRL-1605 copolymer

Chinnaswamy Jagannath, Alice Wells, Medea Mshvildadze, Margaret Olsen, Eliud Sepulveda, Martin Emanuele, Robert L. Hunter, Amitava Dasgupta

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Amikacin is an aminoglycoside which is used in the treatment of infection from gram negative bacteria. Amikacin is also used synergistically with penicillin against gram positive cocci. Amikacin cannot be delivered orally probably due to efflux of drug by P-glycoprotein pump in the brush border of intestine. We studied the possibility of delivering amikacin orally in mice using a copolymer (CRL-1605) as a vehicle. This copolymer inhibits P- glycoprotein pump. Two different doses of amikacin were used (500 mg/kg and 100 mg/kg). The concentration of polymer used was 132 mg/kg. The liquid formulation was fed to mice by gavage and serum amikacin concentrations were estimated after one hour and two hours using flourescence polarization immunoassay. We observed a two fold increase in serum amikacin concentration when amikacin was orally delivered in the presence of CRL-1605 compared to controls (amikacin alone). We conclude that gastrointestinal absorption of amikacin is significantly increased in the presence of CRL-1605 in mice.

Original languageEnglish (US)
Pages (from-to)1733-1738
Number of pages6
JournalLife sciences
Volume64
Issue number19
DOIs
StatePublished - Apr 2 1999

Keywords

  • Amikacin
  • CRL-1605
  • Oral uptake

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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