Serotonin depletion hampers survival and proliferation in neurospheres derived from adult neural stem cells

Jens Benninghoff; Angela Gritti; Matteo Rizzi; Giuseppe Lamorte; Robert J. Schloesser; Angelika Schmitt; Stefanie Robel; Just Genius; Rainald Moessner; Peter Riederer; Husseini K. Manji; Heinz Grunze; Dan Rujescu; Hans Juergen Moeller; Klaus Peter Lesch; Angelo Luigi Vescovi

doi:10.1038/npp.2009.181

Serotonin depletion hampers survival and proliferation in neurospheres derived from adult neural stem cells

Jens Benninghoff, Angela Gritti, Matteo Rizzi, Giuseppe Lamorte, Robert J. Schloesser, Angelika Schmitt, Stefanie Robel, Just Genius, Rainald Moessner, Peter Riederer, Husseini K. Manji, Heinz Grunze, Dan Rujescu, Hans Juergen Moeller, Klaus Peter Lesch, Angelo Luigi Vescovi

Research output: Contribution to journal › Article

33 Scopus citations

Abstract

Serotonin (5-HT) and the serotonergic system have recently been indicated as modulators of adult hippocampal neurogenesis. In this study, we evaluated the role of 5-HT on the functional features in neurospheres derived from adult neural stem cells (ANSC). We cultured neurospheres derived from mouse hippocampus in serum-free medium containing epidermal (EGF) and type-2 fibroblast growth factor (FGF2). Under these conditions ANSC expressed both isoforms of tryptophane-hydroxylase (TPH) and produced 5-HT. Blocking TPH function by para-chlorophenylalanine (PCPA) reduced ANSC proliferation, which was rescued by exogenous 5-HT. 5-HT action on ANSC was mediated predominantly by the serotonin receptor subtype 5-HT1A and, to a lesser extent, through the 5-HT2C (receptor) subtype, as shown by selectively antagonizing these receptors. Finally, we documented a 5-HT-induced increase of ANSC migration activity. In summary, we demonstrated a powerful serotonergic impact on ANSC functional features, which was mainly mediated by 5-HT1A receptors.

Original language	English (US)
Pages (from-to)	893-903
Number of pages	11
Journal	Neuropsychopharmacology
Volume	35
Issue number	4
DOIs	https://doi.org/10.1038/npp.2009.181
State	Published - Mar 2010

Keywords

Brain
Hippocampus
Neural progenitors
Neurogenesis
Serotonin
TPH

ASJC Scopus subject areas

Pharmacology
Psychiatry and Mental health

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10.1038/npp.2009.181

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Benninghoff, J., Gritti, A., Rizzi, M., Lamorte, G., Schloesser, R. J., Schmitt, A., Robel, S., Genius, J., Moessner, R., Riederer, P., Manji, H. K., Grunze, H., Rujescu, D., Moeller, H. J., Lesch, K. P., & Vescovi, A. L. (2010). Serotonin depletion hampers survival and proliferation in neurospheres derived from adult neural stem cells. Neuropsychopharmacology, 35(4), 893-903. https://doi.org/10.1038/npp.2009.181

Serotonin depletion hampers survival and proliferation in neurospheres derived from adult neural stem cells. / Benninghoff, Jens; Gritti, Angela; Rizzi, Matteo; Lamorte, Giuseppe; Schloesser, Robert J.; Schmitt, Angelika; Robel, Stefanie; Genius, Just; Moessner, Rainald; Riederer, Peter; Manji, Husseini K.; Grunze, Heinz; Rujescu, Dan; Moeller, Hans Juergen; Lesch, Klaus Peter; Vescovi, Angelo Luigi.

In: Neuropsychopharmacology, Vol. 35, No. 4, 03.2010, p. 893-903.

Research output: Contribution to journal › Article

Benninghoff, J, Gritti, A, Rizzi, M, Lamorte, G, Schloesser, RJ, Schmitt, A, Robel, S, Genius, J, Moessner, R, Riederer, P, Manji, HK, Grunze, H, Rujescu, D, Moeller, HJ, Lesch, KP & Vescovi, AL 2010, 'Serotonin depletion hampers survival and proliferation in neurospheres derived from adult neural stem cells', Neuropsychopharmacology, vol. 35, no. 4, pp. 893-903. https://doi.org/10.1038/npp.2009.181

Benninghoff, Jens ; Gritti, Angela ; Rizzi, Matteo ; Lamorte, Giuseppe ; Schloesser, Robert J. ; Schmitt, Angelika ; Robel, Stefanie ; Genius, Just ; Moessner, Rainald ; Riederer, Peter ; Manji, Husseini K. ; Grunze, Heinz ; Rujescu, Dan ; Moeller, Hans Juergen ; Lesch, Klaus Peter ; Vescovi, Angelo Luigi. / Serotonin depletion hampers survival and proliferation in neurospheres derived from adult neural stem cells. In: Neuropsychopharmacology. 2010 ; Vol. 35, No. 4. pp. 893-903.

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