Serotonin 1A receptor messenger RNA regulation in the hippocampus after acute stress

Juan F. López; Israel Liberzon; Delia M. Vázquez; Elizabeth A. Young; Stanley J. Watson

doi:10.1016/S0006-3223(98)00224-8

Serotonin 1A receptor messenger RNA regulation in the hippocampus after acute stress

Juan F. López, Israel Liberzon, Delia M. Vázquez, Elizabeth A. Young, Stanley J. Watson

Research output: Contribution to journal › Article › peer-review

74 Scopus citations

Abstract

Background: When rats are subjected to chronic stress for 2 weeks, a significant decrease in hippocampal serotonin (5-HT)(1A) messenger RNA (mRNA) is observed. We wanted to investigate whether stress, administered for shorter periods of time, would result in decreases in 5-HT(1A) gene expression in hippocampus. Methods: In one experiment, rats were either stressed daily for 1 week or implanted with two corticosterone pellets to produce elevated corticosterone levels. In another experiment, rats were subjected to a severe acute stressor and sacrificed 1 day or 1 week after the stressor. Results: We found that 24 hours after the acute stress, rats showed a significant decrease in 5-HT(1A) mRNA levels in CA1 and the dentate gyrus compared to controls. No significant changes in 5-HT(1A) mRNA levels were detected in any of the other groups. Conclusions: Although 1 week of chronic stress is not sufficient to cause significant decreases in hippocampal 5- HT(1A) mRNA levels, a severe and prolonged acute stress is capable of down- regulating, at least transiently, 5-HT(1A) mRNA gene expression in hippocampus.

Original language	English (US)
Pages (from-to)	934-937
Number of pages	4
Journal	Biological Psychiatry
Volume	45
Issue number	7
DOIs	https://doi.org/10.1016/S0006-3223(98)00224-8
State	Published - Apr 1 1999

Keywords

Hippocampus
In situ hybridization
Rat
Receptor
Serotonin
Stress

ASJC Scopus subject areas

Biological Psychiatry

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10.1016/S0006-3223(98)00224-8

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title = "Serotonin 1A receptor messenger RNA regulation in the hippocampus after acute stress",

abstract = "Background: When rats are subjected to chronic stress for 2 weeks, a significant decrease in hippocampal serotonin (5-HT)(1A) messenger RNA (mRNA) is observed. We wanted to investigate whether stress, administered for shorter periods of time, would result in decreases in 5-HT(1A) gene expression in hippocampus. Methods: In one experiment, rats were either stressed daily for 1 week or implanted with two corticosterone pellets to produce elevated corticosterone levels. In another experiment, rats were subjected to a severe acute stressor and sacrificed 1 day or 1 week after the stressor. Results: We found that 24 hours after the acute stress, rats showed a significant decrease in 5-HT(1A) mRNA levels in CA1 and the dentate gyrus compared to controls. No significant changes in 5-HT(1A) mRNA levels were detected in any of the other groups. Conclusions: Although 1 week of chronic stress is not sufficient to cause significant decreases in hippocampal 5- HT(1A) mRNA levels, a severe and prolonged acute stress is capable of down- regulating, at least transiently, 5-HT(1A) mRNA gene expression in hippocampus.",

keywords = "Hippocampus, In situ hybridization, Rat, Receptor, Serotonin, Stress",

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language = "English (US)",

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T1 - Serotonin 1A receptor messenger RNA regulation in the hippocampus after acute stress

AU - López, Juan F.

AU - Liberzon, Israel

AU - Vázquez, Delia M.

AU - Young, Elizabeth A.

AU - Watson, Stanley J.

PY - 1999/4/1

Y1 - 1999/4/1

N2 - Background: When rats are subjected to chronic stress for 2 weeks, a significant decrease in hippocampal serotonin (5-HT)(1A) messenger RNA (mRNA) is observed. We wanted to investigate whether stress, administered for shorter periods of time, would result in decreases in 5-HT(1A) gene expression in hippocampus. Methods: In one experiment, rats were either stressed daily for 1 week or implanted with two corticosterone pellets to produce elevated corticosterone levels. In another experiment, rats were subjected to a severe acute stressor and sacrificed 1 day or 1 week after the stressor. Results: We found that 24 hours after the acute stress, rats showed a significant decrease in 5-HT(1A) mRNA levels in CA1 and the dentate gyrus compared to controls. No significant changes in 5-HT(1A) mRNA levels were detected in any of the other groups. Conclusions: Although 1 week of chronic stress is not sufficient to cause significant decreases in hippocampal 5- HT(1A) mRNA levels, a severe and prolonged acute stress is capable of down- regulating, at least transiently, 5-HT(1A) mRNA gene expression in hippocampus.

AB - Background: When rats are subjected to chronic stress for 2 weeks, a significant decrease in hippocampal serotonin (5-HT)(1A) messenger RNA (mRNA) is observed. We wanted to investigate whether stress, administered for shorter periods of time, would result in decreases in 5-HT(1A) gene expression in hippocampus. Methods: In one experiment, rats were either stressed daily for 1 week or implanted with two corticosterone pellets to produce elevated corticosterone levels. In another experiment, rats were subjected to a severe acute stressor and sacrificed 1 day or 1 week after the stressor. Results: We found that 24 hours after the acute stress, rats showed a significant decrease in 5-HT(1A) mRNA levels in CA1 and the dentate gyrus compared to controls. No significant changes in 5-HT(1A) mRNA levels were detected in any of the other groups. Conclusions: Although 1 week of chronic stress is not sufficient to cause significant decreases in hippocampal 5- HT(1A) mRNA levels, a severe and prolonged acute stress is capable of down- regulating, at least transiently, 5-HT(1A) mRNA gene expression in hippocampus.

KW - Hippocampus

KW - In situ hybridization

KW - Rat

KW - Receptor

KW - Serotonin

KW - Stress

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Serotonin 1A receptor messenger RNA regulation in the hippocampus after acute stress

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