SERCA2a and mitochondrial cytochrome oxidase expression are increased in hearts of exercise-trained old rats

Charlotte A. Tate; Thorunn Helgason; Michael F. Hyek; R. Perley Mcbride; Minhui Chen; Michelle A. Richardson; George E. Taffet

doi:10.1152/ajpheart.1996.271.1.h68

SERCA2a and mitochondrial cytochrome oxidase expression are increased in hearts of exercise-trained old rats

Charlotte A. Tate, Thorunn Helgason, Michael F. Hyek, R. Perley Mcbride, Minhui Chen, Michelle A. Richardson, George E. Taffet

Research output: Contribution to journal › Article › peer-review

70 Scopus citations

Abstract

Aging of rats results in slower activities of calcium transport by cardiac calcium adenosinetriphosphatase (ATPase) of the sarcoplasmic reticulum (SR) and mitochondrial cytochrome oxidase (COX). These enzyme activities are faster after exercise training of previously sedentary old rats. Our purpose was to determine whether the expression of the genes encoding SR calcium ATPase (SERCA2a) or COX is altered by exercise training. Old (24-mo-old) male Fischer 344 rats were assigned to SO (sedentary old) or EO (exercised old) groups and compared with younger (12-mo-old) sedentary rats (SM/. EO rats were trained on a treadmill for 8-10 wk. SERCA2a and COX mRNAs were lower (P < 0.05) in SO compared with SM and EO, whereas glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and cardiac α-actin mRNAs were similar across groups. The immunoreactive protein contents of cardiac calcium ATPase, cytochrome ε, sarcomeric actin, and GAPDH followed the changes, when observed, in mRNA contents. Thus pretranslational mechanisms may be modified in some genes during aging and exercise training of previously sedentary old rats.

Original language	English (US)
Pages (from-to)	H68-H72
Journal	American Journal of Physiology - Heart and Circulatory Physiology
Volume	271
Issue number	1 40-1
DOIs	https://doi.org/10.1152/ajpheart.1996.271.1.h68
State	Published - Jul 1996

Keywords

Fischer 344 rats
SERCA2a gene
actin
mitochondrial cytochrome oxidase
sarcoplasmic reticulum calcium adenosinetriphosphatase

ASJC Scopus subject areas

Physiology
Cardiology and Cardiovascular Medicine
Physiology (medical)

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10.1152/ajpheart.1996.271.1.h68

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title = "SERCA2a and mitochondrial cytochrome oxidase expression are increased in hearts of exercise-trained old rats",

abstract = "Aging of rats results in slower activities of calcium transport by cardiac calcium adenosinetriphosphatase (ATPase) of the sarcoplasmic reticulum (SR) and mitochondrial cytochrome oxidase (COX). These enzyme activities are faster after exercise training of previously sedentary old rats. Our purpose was to determine whether the expression of the genes encoding SR calcium ATPase (SERCA2a) or COX is altered by exercise training. Old (24-mo-old) male Fischer 344 rats were assigned to SO (sedentary old) or EO (exercised old) groups and compared with younger (12-mo-old) sedentary rats (SM/. EO rats were trained on a treadmill for 8-10 wk. SERCA2a and COX mRNAs were lower (P < 0.05) in SO compared with SM and EO, whereas glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and cardiac α-actin mRNAs were similar across groups. The immunoreactive protein contents of cardiac calcium ATPase, cytochrome ε, sarcomeric actin, and GAPDH followed the changes, when observed, in mRNA contents. Thus pretranslational mechanisms may be modified in some genes during aging and exercise training of previously sedentary old rats.",

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AU - Hyek, Michael F.

AU - Mcbride, R. Perley

AU - Chen, Minhui

AU - Richardson, Michelle A.

AU - Taffet, George E.

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SERCA2a and mitochondrial cytochrome oxidase expression are increased in hearts of exercise-trained old rats

Abstract

Keywords

ASJC Scopus subject areas

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