SERCA2a and mitochondrial cytochrome oxidase expression are increased in hearts of exercise-trained old rats

Charlotte A. Tate, Thorunn Helgason, Michael F. Hyek, R. Perley Mcbride, Minhui Chen, Michelle A. Richardson, George E. Taffet

Research output: Contribution to journalArticle

63 Scopus citations

Abstract

Aging of rats results in slower activities of calcium transport by cardiac calcium adenosinetriphosphatase (ATPase) of the sarcoplasmic reticulum (SR) and mitochondrial cytochrome oxidase (COX). These enzyme activities are faster after exercise training of previously sedentary old rats. Our purpose was to determine whether the expression of the genes encoding SR calcium ATPase (SERCA2a) or COX is altered by exercise training. Old (24-mo-old) male Fischer 344 rats were assigned to SO (sedentary old) or EO (exercised old) groups and compared with younger (12-mo-old) sedentary rats (SM/. EO rats were trained on a treadmill for 8-10 wk. SERCA2a and COX mRNAs were lower (P < 0.05) in SO compared with SM and EO, whereas glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and cardiac α-actin mRNAs were similar across groups. The immunoreactive protein contents of cardiac calcium ATPase, cytochrome ε, sarcomeric actin, and GAPDH followed the changes, when observed, in mRNA contents. Thus pretranslational mechanisms may be modified in some genes during aging and exercise training of previously sedentary old rats.

Original languageEnglish (US)
Pages (from-to)H68-H72
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume271
Issue number1 40-1
DOIs
StatePublished - Jul 1996

Keywords

  • Fischer 344 rats
  • SERCA2a gene
  • actin
  • mitochondrial cytochrome oxidase
  • sarcoplasmic reticulum calcium adenosinetriphosphatase

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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