Sequence Analysis, Tissue Expression and Chromosomal Localization of a Mouse Secreted Superoxide Dismutase Gene

Jun Gyo Suh; Setsuo Takai; Toshiyuki Yamanishi; Tateki Kikuchi; Rodney J. Folz; Kohichi Tanaka; Yang Seok Oh; Keiji Wada

doi:10.1016/s1016-8478(23)13281-x

Sequence Analysis, Tissue Expression and Chromosomal Localization of a Mouse Secreted Superoxide Dismutase Gene

Jun Gyo Suh, Setsuo Takai, Toshiyuki Yamanishi, Tateki Kikuchi, Rodney J. Folz, Kohichi Tanaka, Yang Seok Oh, Keiji Wada

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Abstract

Using a reverse transcriptase polymerase chain reaction (RT-PCR), a complement DNA encoding secreted superoxide dismutase (s-SOD) of a mouse kidney has been isolated and the nucleotide sequence was determined. The deduced amino acid sequence of mouse s-SOD cDNA shares 79% identity with the rat seminal SOD sequence and 61% identity with the human SOD3 sequence. Northern blot analysis showed that mouse s-SOD is intensely expressed in the kidney and lung tissues and detectable in other tested tissues, including the brain. The mouse s-SOD gene was assigned to chromosome 5 using fluorescence in situ hybridization analysis and PCR analysis of mouse/hamster hybrid cells.

Original language	English (US)
Pages (from-to)	204-207
Number of pages	4
Journal	Molecules and Cells
Volume	7
Issue number	2
DOIs	https://doi.org/10.1016/s1016-8478(23)13281-x
State	Published - Apr 30 1997

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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title = "Sequence Analysis, Tissue Expression and Chromosomal Localization of a Mouse Secreted Superoxide Dismutase Gene",

abstract = "Using a reverse transcriptase polymerase chain reaction (RT-PCR), a complement DNA encoding secreted superoxide dismutase (s-SOD) of a mouse kidney has been isolated and the nucleotide sequence was determined. The deduced amino acid sequence of mouse s-SOD cDNA shares 79% identity with the rat seminal SOD sequence and 61% identity with the human SOD3 sequence. Northern blot analysis showed that mouse s-SOD is intensely expressed in the kidney and lung tissues and detectable in other tested tissues, including the brain. The mouse s-SOD gene was assigned to chromosome 5 using fluorescence in situ hybridization analysis and PCR analysis of mouse/hamster hybrid cells.",

author = "Suh, {Jun Gyo} and Setsuo Takai and Toshiyuki Yamanishi and Tateki Kikuchi and Folz, {Rodney J.} and Kohichi Tanaka and Oh, {Yang Seok} and Keiji Wada",

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T1 - Sequence Analysis, Tissue Expression and Chromosomal Localization of a Mouse Secreted Superoxide Dismutase Gene

AU - Suh, Jun Gyo

AU - Takai, Setsuo

AU - Yamanishi, Toshiyuki

AU - Kikuchi, Tateki

AU - Folz, Rodney J.

AU - Tanaka, Kohichi

AU - Oh, Yang Seok

AU - Wada, Keiji

PY - 1997/4/30

Y1 - 1997/4/30

N2 - Using a reverse transcriptase polymerase chain reaction (RT-PCR), a complement DNA encoding secreted superoxide dismutase (s-SOD) of a mouse kidney has been isolated and the nucleotide sequence was determined. The deduced amino acid sequence of mouse s-SOD cDNA shares 79% identity with the rat seminal SOD sequence and 61% identity with the human SOD3 sequence. Northern blot analysis showed that mouse s-SOD is intensely expressed in the kidney and lung tissues and detectable in other tested tissues, including the brain. The mouse s-SOD gene was assigned to chromosome 5 using fluorescence in situ hybridization analysis and PCR analysis of mouse/hamster hybrid cells.

AB - Using a reverse transcriptase polymerase chain reaction (RT-PCR), a complement DNA encoding secreted superoxide dismutase (s-SOD) of a mouse kidney has been isolated and the nucleotide sequence was determined. The deduced amino acid sequence of mouse s-SOD cDNA shares 79% identity with the rat seminal SOD sequence and 61% identity with the human SOD3 sequence. Northern blot analysis showed that mouse s-SOD is intensely expressed in the kidney and lung tissues and detectable in other tested tissues, including the brain. The mouse s-SOD gene was assigned to chromosome 5 using fluorescence in situ hybridization analysis and PCR analysis of mouse/hamster hybrid cells.

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Sequence Analysis, Tissue Expression and Chromosomal Localization of a Mouse Secreted Superoxide Dismutase Gene

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