Sequence Analysis, Tissue Expression and Chromosomal Localization of a Mouse Secreted Superoxide Dismutase Gene

Jun Gyo Suh, Setsuo Takai, Toshiyuki Yamanishi, Tateki Kikuchi, Rodney J. Folz, Kohichi Tanaka, Yang Seok Oh, Keiji Wada

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Using a reverse transcriptase polymerase chain reaction (RT-PCR), a complement DNA encoding secreted superoxide dismutase (s-SOD) of a mouse kidney has been isolated and the nucleotide sequence was determined. The deduced amino acid sequence of mouse s-SOD cDNA shares 79% identity with the rat seminal SOD sequence and 61% identity with the human SOD3 sequence. Northern blot analysis showed that mouse s-SOD is intensely expressed in the kidney and lung tissues and detectable in other tested tissues, including the brain. The mouse s-SOD gene was assigned to chromosome 5 using fluorescence in situ hybridization analysis and PCR analysis of mouse/hamster hybrid cells.

Original languageEnglish (US)
Pages (from-to)204-207
Number of pages4
JournalMolecules and Cells
Volume7
Issue number2
StatePublished - Apr 30 1997

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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