Separation of apolipoproteins-a-i and a-ii by ion-paired reversed-phase high-performance liquid chromatography

W. S. Hancock; H. J. Pownall; A. M. Gotto; J. T. Sparrow

doi:10.1016/S0021-9673(00)82357-2

Separation of apolipoproteins-a-i and a-ii by ion-paired reversed-phase high-performance liquid chromatography

W. S. Hancock, H. J. Pownall, A. M. Gotto, J. T. Sparrow

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

The separation of apolipoproteins A-I and A-II, isolated from human high density lipoproteins (HDL), was achieved on either a μBondapak-alkylphenyl or Zorbax-C₈ column. The mobile phase consisted of 1% triethylammonium phosphate, pH 3.2 with a shallow linear gradient of acetonitrile. These conditions were used to separate mixtures of apolipoproteins A-I and A-II and a mixture of the apoprotein constituents of HDL.

Original language	English (US)
Pages (from-to)	285-293
Number of pages	9
Journal	Journal of Chromatography A
Volume	216
Issue number	C
DOIs	https://doi.org/10.1016/S0021-9673(00)82357-2
State	Published - Oct 30 1981

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

Divisions

Endocrinology, Diabetes and Metabolism

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10.1016/S0021-9673(00)82357-2

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title = "Separation of apolipoproteins-a-i and a-ii by ion-paired reversed-phase high-performance liquid chromatography",

abstract = "The separation of apolipoproteins A-I and A-II, isolated from human high density lipoproteins (HDL), was achieved on either a μBondapak-alkylphenyl or Zorbax-C8 column. The mobile phase consisted of 1\% triethylammonium phosphate, pH 3.2 with a shallow linear gradient of acetonitrile. These conditions were used to separate mixtures of apolipoproteins A-I and A-II and a mixture of the apoprotein constituents of HDL.",

author = "Hancock, \{W. S.\} and Pownall, \{H. J.\} and Gotto, \{A. M.\} and Sparrow, \{J. T.\}",

note = "Funding Information: tional Heart and Blood Vessel Research arid Demonstration Center, Baylor College of Medicine, a grant supported project of the National Heart, Lung and Blood Institute, National Institutes of Health Grant No. 17269. The research work was ably supported by Mr. Stanley Moore (amino acid analysis). Funding Information: The authors wish to acknowledge support from the New Zealand Heart Foundation (Grant Number 77/200) and the American Heart Association. This material was developed by the Atherosclerosis, Lipids and Lipoproteins Section of the Na-",

year = "1981",

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doi = "10.1016/S0021-9673(00)82357-2",

language = "English (US)",

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pages = "285--293",

journal = "Journal of Chromatography A",

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T1 - Separation of apolipoproteins-a-i and a-ii by ion-paired reversed-phase high-performance liquid chromatography

AU - Hancock, W. S.

AU - Pownall, H. J.

AU - Gotto, A. M.

AU - Sparrow, J. T.

N1 - Funding Information: tional Heart and Blood Vessel Research arid Demonstration Center, Baylor College of Medicine, a grant supported project of the National Heart, Lung and Blood Institute, National Institutes of Health Grant No. 17269. The research work was ably supported by Mr. Stanley Moore (amino acid analysis). Funding Information: The authors wish to acknowledge support from the New Zealand Heart Foundation (Grant Number 77/200) and the American Heart Association. This material was developed by the Atherosclerosis, Lipids and Lipoproteins Section of the Na-

PY - 1981/10/30

Y1 - 1981/10/30

N2 - The separation of apolipoproteins A-I and A-II, isolated from human high density lipoproteins (HDL), was achieved on either a μBondapak-alkylphenyl or Zorbax-C8 column. The mobile phase consisted of 1% triethylammonium phosphate, pH 3.2 with a shallow linear gradient of acetonitrile. These conditions were used to separate mixtures of apolipoproteins A-I and A-II and a mixture of the apoprotein constituents of HDL.

AB - The separation of apolipoproteins A-I and A-II, isolated from human high density lipoproteins (HDL), was achieved on either a μBondapak-alkylphenyl or Zorbax-C8 column. The mobile phase consisted of 1% triethylammonium phosphate, pH 3.2 with a shallow linear gradient of acetonitrile. These conditions were used to separate mixtures of apolipoproteins A-I and A-II and a mixture of the apoprotein constituents of HDL.

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U2 - 10.1016/S0021-9673(00)82357-2

DO - 10.1016/S0021-9673(00)82357-2

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JF - Journal of Chromatography A

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ER -

Separation of apolipoproteins-a-i and a-ii by ion-paired reversed-phase high-performance liquid chromatography

Abstract

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