Separation of apolipoproteins-a-i and a-ii by ion-paired reversed-phase high-performance liquid chromatography

W. S. Hancock, Henry J. Pownall, Antonio Gotto, J. T. Sparrow

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The separation of apolipoproteins A-I and A-II, isolated from human high density lipoproteins (HDL), was achieved on either a μBondapak-alkylphenyl or Zorbax-C8 column. The mobile phase consisted of 1% triethylammonium phosphate, pH 3.2 with a shallow linear gradient of acetonitrile. These conditions were used to separate mixtures of apolipoproteins A-I and A-II and a mixture of the apoprotein constituents of HDL.

Original languageEnglish (US)
Pages (from-to)285-293
Number of pages9
JournalJournal of Chromatography A
Volume216
Issue numberC
DOIs
StatePublished - Oct 30 1981

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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