Sensitive detection of norovirus using phage nanoparticle reporters in lateral-flow assay

Anna E V Hagström, Gavin Garvey, Andrew S. Paterson, Sagar Dhamane, Meena Adhikari, Mary K. Estes, Ulrich Strych, Katerina Kourentzi, Robert L. Atmar, Richard C. Willson

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Noroviruses are recognized worldwide as the principal cause of acute, non-bacterial gastroenteritis, resulting in 19-21 million cases of disease every year in the United States. Noroviruses have a very low infectious dose, a short incubation period, high resistance to traditional disinfection techniques and multiple modes of transmission, making early, point-of-care detection essential for controlling the spread of the disease. The traditional diagnostic tools, electron microscopy, RT-PCR and ELISA require sophisticated and expensive instrumentation, and are considered too laborious and slow to be useful during severe outbreaks. In this paper we describe the development of a new, rapid and sensitive lateral-flow assay using labeled phage particles for the detection of the prototypical norovirus GI.1 (Norwalk), with a limit of detection of 107 virus-like particles per mL, one hundredfold lower than a conventional gold nanoparticle lateral-flow assay using the same antibody pair.

Original languageEnglish (US)
Article number0126571
JournalPLoS ONE
Volume10
Issue number5
DOIs
StatePublished - May 15 2015

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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