TY - JOUR
T1 - Sensitive detection of norovirus using phage nanoparticle reporters in lateral-flow assay
AU - Hagström, Anna E V
AU - Garvey, Gavin
AU - Paterson, Andrew S.
AU - Dhamane, Sagar
AU - Adhikari, Meena
AU - Estes, Mary K.
AU - Strych, Ulrich
AU - Kourentzi, Katerina
AU - Atmar, Robert L.
AU - Willson, Richard C.
N1 - Publisher Copyright:
© 2015 Hagström et al.
PY - 2015/5/15
Y1 - 2015/5/15
N2 - Noroviruses are recognized worldwide as the principal cause of acute, non-bacterial gastroenteritis, resulting in 19-21 million cases of disease every year in the United States. Noroviruses have a very low infectious dose, a short incubation period, high resistance to traditional disinfection techniques and multiple modes of transmission, making early, point-of-care detection essential for controlling the spread of the disease. The traditional diagnostic tools, electron microscopy, RT-PCR and ELISA require sophisticated and expensive instrumentation, and are considered too laborious and slow to be useful during severe outbreaks. In this paper we describe the development of a new, rapid and sensitive lateral-flow assay using labeled phage particles for the detection of the prototypical norovirus GI.1 (Norwalk), with a limit of detection of 107 virus-like particles per mL, one hundredfold lower than a conventional gold nanoparticle lateral-flow assay using the same antibody pair.
AB - Noroviruses are recognized worldwide as the principal cause of acute, non-bacterial gastroenteritis, resulting in 19-21 million cases of disease every year in the United States. Noroviruses have a very low infectious dose, a short incubation period, high resistance to traditional disinfection techniques and multiple modes of transmission, making early, point-of-care detection essential for controlling the spread of the disease. The traditional diagnostic tools, electron microscopy, RT-PCR and ELISA require sophisticated and expensive instrumentation, and are considered too laborious and slow to be useful during severe outbreaks. In this paper we describe the development of a new, rapid and sensitive lateral-flow assay using labeled phage particles for the detection of the prototypical norovirus GI.1 (Norwalk), with a limit of detection of 107 virus-like particles per mL, one hundredfold lower than a conventional gold nanoparticle lateral-flow assay using the same antibody pair.
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U2 - 10.1371/journal.pone.0126571
DO - 10.1371/journal.pone.0126571
M3 - Article
C2 - 25978622
AN - SCOPUS:84929377736
VL - 10
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 5
M1 - 0126571
ER -