TY - JOUR
T1 - Sensing Temperature in Vitro and in Cells Using a BODIPY Molecular Probe
AU - Ogle, Meredith M.
AU - Smith McWilliams, Ashleigh D.
AU - Ware, Matthew J.
AU - Curley, Steven A.
AU - Corr, Stuart J.
AU - Martí, Angel A.
N1 - Publisher Copyright:
Copyright © 2019 American Chemical Society.
PY - 2019/5/8
Y1 - 2019/5/8
N2 - Boron dipyrromethene (BODIPY) molecular rotors have shown sensitivity toward viscosity, polarity, and temperature. Here, we report a 1,3,5,7-tetramethyl-8-phenyl-BODIPY modified with a polyethylene glycol (PEG) chain, for temperature sensing and live cell imaging. This new PEG-BODIPY dye presents an increase in nonradiative decay as temperature increases, which directly influences its lifetime. This change in lifetime is dependent on changes in both temperature and viscosity at low viscosity values, but is only dependent on temperature at high viscosity values. The dependence of fluorescence lifetime with temperature allows for temperature monitoring in vitro and in cells, with sub degree resolution. When in contact with cells, the PEG-BODIPY spontaneously penetrates and stains the cell but not the nucleus. Furthermore, no significant cell toxicity was found even at 100 μM concentration. Using fluorescence lifetime imaging microscopy (FLIM), we were able to observe the changes in the lifetime of PEG-BODIPY within the cell at different temperatures. The use of FLIM and molecular probes such as PEG-BODIPY can provide important information about cellular temperature and heat dissipation upon medically relevant stimuli, such as radiofrequency ablation and photodynamic therapy.
AB - Boron dipyrromethene (BODIPY) molecular rotors have shown sensitivity toward viscosity, polarity, and temperature. Here, we report a 1,3,5,7-tetramethyl-8-phenyl-BODIPY modified with a polyethylene glycol (PEG) chain, for temperature sensing and live cell imaging. This new PEG-BODIPY dye presents an increase in nonradiative decay as temperature increases, which directly influences its lifetime. This change in lifetime is dependent on changes in both temperature and viscosity at low viscosity values, but is only dependent on temperature at high viscosity values. The dependence of fluorescence lifetime with temperature allows for temperature monitoring in vitro and in cells, with sub degree resolution. When in contact with cells, the PEG-BODIPY spontaneously penetrates and stains the cell but not the nucleus. Furthermore, no significant cell toxicity was found even at 100 μM concentration. Using fluorescence lifetime imaging microscopy (FLIM), we were able to observe the changes in the lifetime of PEG-BODIPY within the cell at different temperatures. The use of FLIM and molecular probes such as PEG-BODIPY can provide important information about cellular temperature and heat dissipation upon medically relevant stimuli, such as radiofrequency ablation and photodynamic therapy.
UR - http://www.scopus.com/inward/record.url?scp=85071708542&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85071708542&partnerID=8YFLogxK
U2 - 10.1021/acs.jpcb.9b04384
DO - 10.1021/acs.jpcb.9b04384
M3 - Article
C2 - 31429279
AN - SCOPUS:85071708542
SN - 1520-6106
VL - 123
SP - 7282
EP - 7289
JO - Journal of Physical Chemistry B
JF - Journal of Physical Chemistry B
IS - 34
ER -