Sensing of Bacillus Subtilis spores with peptide functionalized microcantilevers

Babita Dhayal, R. Reifenberger, Walter A. Henne, Derek D. Doorneweerd, Philip S. Low

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Short peptide ligands were synthesized to selectively capture spores using microcantilever arrays. Our initial studies focused on the capture of Bacillus subtilis (a stimulant of Bacillus anthracis) spores in liquids. A peptide-ligand-functionalized microcantilever array was mounted on a flow cell filled with a B. subtilis suspension while cantilever deflection was monitored. Also, fifth mode resonant frequency measurements were performed before and after dipping a peptide-ligand-functionalized microcantilever array in a static B. subtilis solution. Both techniques showed selective binding to functionalized cantilevers when compared to arrays functionalized with a similar control (non-binding) peptide. Conclusive confirmation of selective binding was obtained by subsequent examination of the microcantilever arrays under a dark field microscope. The concentration sensitivity, shelf-life of the binding ligands, and their binding efficiency were studied as well.

Original languageEnglish (US)
Title of host publication2006 6th IEEE Conference on Nanotechnology, IEEE-NANO 2006
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages787-789
Number of pages3
ISBN (Print)1424400783, 9781424400782
DOIs
StatePublished - 2006
Event2006 6th IEEE Conference on Nanotechnology, IEEE-NANO 2006 - Cincinnati, OH, United States
Duration: Jun 17 2006Jun 20 2006

Publication series

Name2006 6th IEEE Conference on Nanotechnology, IEEE-NANO 2006
Volume2

Other

Other2006 6th IEEE Conference on Nanotechnology, IEEE-NANO 2006
CountryUnited States
CityCincinnati, OH
Period6/17/066/20/06

Keywords

  • Anthrax
  • Microcantilevers
  • Nano-bio-sensors
  • Peptides

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Materials Science(all)

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