Self-Assembly of Copper–DNAzyme Nanohybrids for Dual-Catalytic Tumor Therapy

Congzhi Liu, Yaoxuan Chen, Jian Zhao, Yong Wang, Yulei Shao, Zhanjun Gu, Lele Li, Yuliang Zhao

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Despite the great efforts of using DNAzyme for gene therapy, its clinical success is limited by the lack of simple delivery systems and limited anticancer efficacy. Here, we develop a simple approach for the synthesis of hybrid nanostructures that exclusively consist of DNAzyme and Cu2+ with ultra-high loading capacity. The Cu–DNAzyme nanohybrids allow to effectively co-deliver DNAzyme and Cu2+ into cancer cells for combinational catalytic therapy. The released Cu2+ can be reduced to Cu+ by glutathione and then catalyze endogenous H2O2 to form cytotoxic hydroxyl radicals for chemodynamic therapy (CDT), while the 10–23 DNAzyme enables the catalytic cleavage of VEGFR2 mRNA and activates gene silencing for gene therapy. We demonstrate that the system can efficiently accumulate in the tumor and exhibit amplified cascade antitumor effects with negligible systemic toxicity. Our work paves an extremely simple way to integrate DNAzyme with CDT for the dual-catalytic tumor treatment.

Original languageEnglish (US)
Pages (from-to)14324-14328
Number of pages5
JournalAngewandte Chemie - International Edition
Volume60
Issue number26
DOIs
StatePublished - Jun 21 2021

Keywords

  • DNAzyme
  • Fenton-like reaction
  • chemodynamic therapy
  • gene therapy
  • nanohybrid

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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