SELEX-on-a-chip: Microchip integration of the sol-gel derived affinity column for monitoring RNA-protein interaction

Seung Min Park; Jiyoung Ahn; Minjoung Jo; Soyoun Kim; John T. Lis; Harold G. Craighead

SELEX-on-a-chip: Microchip integration of the sol-gel derived affinity column for monitoring RNA-protein interaction

Seung Min Park, Jiyoung Ahn, Minjoung Jo, Soyoun Kim, John T. Lis, Harold G. Craighead

Houston Methodist

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A microchip that integrates the sol-gel affinity column of a SELEX system is de-veloped. This microchip includes electric heaters for individual heat elution from sol-gels which contain target molecules. To test the microfluidic system for SELEX compatibility, a known aptamer targeting TBP was delivered, then eluted from sol-gels and collected for RT-PCR. We also demonstrated the multiplex SELEX in this microfluidic system by well-defined aptamers that bind either TBP and TFIIB. We could specifically bind and elute the corresponding aptamers from each target pro-tein sol-gel. Comparing to the conventional SELEX, our microfluidic SELEX sys-tem improved cycle efficiency by 2 cycles.

Original language	English
Title of host publication	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
Publisher	Chemical and Biological Microsystems Society
Pages	1087-1089
Number of pages	3
State	Published - Jan 1 2008
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: Oct 12 2008 → Oct 16 2008

Other

Other	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	10/12/08 → 10/16/08

Keywords

Aptamer
Microheater
SELEX
Sol-gel

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

Park, S. M., Ahn, J., Jo, M., Kim, S., Lis, J. T., & Craighead, H. G. (2008). SELEX-on-a-chip: Microchip integration of the sol-gel derived affinity column for monitoring RNA-protein interaction. In 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference (pp. 1087-1089). Chemical and Biological Microsystems Society.

SELEX-on-a-chip: Microchip integration of the sol-gel derived affinity column for monitoring RNA-protein interaction. / Park, Seung Min; Ahn, Jiyoung; Jo, Minjoung et al.
12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, 2008. p. 1087-1089.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Park, SM, Ahn, J, Jo, M, Kim, S, Lis, JT & Craighead, HG 2008, SELEX-on-a-chip: Microchip integration of the sol-gel derived affinity column for monitoring RNA-protein interaction. in 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, pp. 1087-1089, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States, 10/12/08.

Park, Seung Min ; Ahn, Jiyoung ; Jo, Minjoung et al. / SELEX-on-a-chip : Microchip integration of the sol-gel derived affinity column for monitoring RNA-protein interaction. 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, 2008. pp. 1087-1089

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abstract = "A microchip that integrates the sol-gel affinity column of a SELEX system is de-veloped. This microchip includes electric heaters for individual heat elution from sol-gels which contain target molecules. To test the microfluidic system for SELEX compatibility, a known aptamer targeting TBP was delivered, then eluted from sol-gels and collected for RT-PCR. We also demonstrated the multiplex SELEX in this microfluidic system by well-defined aptamers that bind either TBP and TFIIB. We could specifically bind and elute the corresponding aptamers from each target pro-tein sol-gel. Comparing to the conventional SELEX, our microfluidic SELEX sys-tem improved cycle efficiency by 2 cycles.",

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AU - Kim, Soyoun

AU - Lis, John T.

AU - Craighead, Harold G.

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KW - Microheater

KW - SELEX

KW - Sol-gel

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BT - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference

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SELEX-on-a-chip: Microchip integration of the sol-gel derived affinity column for monitoring RNA-protein interaction

Abstract

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Keywords

ASJC Scopus subject areas

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