SELEX-on-a-chip: Microchip integration of the sol-gel derived affinity column for monitoring RNA-protein interaction

Seung Min Park, Jiyoung Ahn, Minjoung Jo, Soyoun Kim, John T. Lis, Harold G. Craighead

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A microchip that integrates the sol-gel affinity column of a SELEX system is de-veloped. This microchip includes electric heaters for individual heat elution from sol-gels which contain target molecules. To test the microfluidic system for SELEX compatibility, a known aptamer targeting TBP was delivered, then eluted from sol-gels and collected for RT-PCR. We also demonstrated the multiplex SELEX in this microfluidic system by well-defined aptamers that bind either TBP and TFIIB. We could specifically bind and elute the corresponding aptamers from each target pro-tein sol-gel. Comparing to the conventional SELEX, our microfluidic SELEX sys-tem improved cycle efficiency by 2 cycles.

Original languageEnglish
Title of host publication12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
PublisherChemical and Biological Microsystems Society
Pages1087-1089
Number of pages3
StatePublished - Jan 1 2008
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: Oct 12 2008Oct 16 2008

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period10/12/0810/16/08

Keywords

  • Aptamer
  • Microheater
  • SELEX
  • Sol-gel

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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