Selective binding and enrichment for low-molecular weight biomarker molecules in human plasma after exposure to nanoporous silica particles

Rosa Terracciano, Marco Gaspari, Flaviano Testa, Luigi Pasqua, Pierosandro Tagliaferri, Mark Ming Cheng Cheng, A. Jasper Nijdam, Emanuel F. Petricoin, Lance A. Liotta, Giovanni Cuda, Mauro Ferrari, Salvatore Venuta

Research output: Contribution to journalArticle

82 Scopus citations

Abstract

The present manuscript describes a biomarker capturing strategy based on nanoporous silica particles. The method is shown to enrich the yield of species in the low-molecular weight proteome (LMWP), allowing detection of small peptides in the low-nanomolar range. Plasma samples were exposed to the silica particles, and the captured molecular species were profiled using MALDI-TOF. Mass spectra of the silica-treated human plasma samples showed a significant enrichment in MALDI-TOF protein profiles in the LMWP. Preliminary results indicated good level of reproducibility in plasma profiles with CVs on peak heights ranging from 6.3 to 14.7%. The MALDI-TOF signature changed significantly when the characteristics of the nanoporous silica were altered. The facile sample pretreatment before MS analysis, coupled to the potential for tailoring the surface properties of silica supports, hold promise for improving the recovery of low-abundance serum biomarkers.

Original languageEnglish (US)
Pages (from-to)3243-3250
Number of pages8
JournalProteomics
Volume6
Issue number11
DOIs
StatePublished - Jun 2006

Keywords

  • Biomarkers
  • Early detection
  • Human plasma
  • Mass spectrometry
  • Nanotechnology

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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