Selective antibody blockade of lymphocyte migration to mucosal sites and mast cell adhesion

Aiyappa Palecanda, Jean S. Marshall, Xian Li, Michael J. Briskin, Thomas B. Issekutz

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The integrins α4β7 and α4β1 mediate adhesion to the mucosal addressin cell adhesion molecule-1 (MAdCAM-I) and the vascular cell adhesion molecule-1 (VCAM-1) and are important in T cell and allergic inflammatory reactions in the rat. The relative contributions of α4β7 and α4β1 in these reactions is unknown. To examine the role of α4β7 in the rat a new mAb, TA-6, was developed. TA-6 inhibited adhesion to MAdCAM-1 but not to VCAM-1, a characteristic of α4β7 adhesion, and immunofluorescence and immunoprecipitation studies were compatible with binding to α4β7. TA-6 blocked rat lymphocyte adhesion to mesenteric lymph nodes and T cell migration to mucosal lymphoid tissues and it bound to rat mucosal mast cells. TA-6 did not inhibit lymphocyte adhesion to peripheral lymph nodes and T cell migration to peripheral lymphoid tissues or cutaneous inflammatory sites, and was not expressed on connective tissue mast cells.

Original languageEnglish (US)
Pages (from-to)649-657
Number of pages9
JournalJournal of Leukocyte Biology
Volume65
Issue number5
DOIs
StatePublished - 1999

Keywords

  • Immunofluorescence
  • Immunoprecipitation
  • Integrins
  • MAdCAM-1
  • VCAM-1

ASJC Scopus subject areas

  • Cell Biology

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