Selection of DNA aptamers against rat liver X receptors

Ioana Surugiu-Wärnmark, Anette Wärnmark, Gudrun Toresson, Jan Åke Gustafsson, Leif Bülow

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Liver X receptors alpha and beta (LXRα; LXRβ) are members of the nuclear hormone receptor superfamily of ligand-activated transcription factors. LXRs play an important role in the reverse cholesterol transport and govern the expression of many of the proteins that are indispensable for the regulation of normal cholesterol levels in the body. SELEX, an in vitro selection technology, was used on a single stranded DNA library harboring a 12 randomized nucleotide sequence in order to isolate aptamers showing affinity for LXRα. Enzyme-linked assays and surface plasmon resonance measurements showed that the selected aptamers had strong affinities for LXRα with apparent dissociation constants, Kds, in nanomolar range. All clones carried CG-repeats, indicating a probability for a similar manner of binding to LXRα. Very high cross-reactivities were observed when testing the aptamers with LXRβ (up to 700%) and RXRα (up to 50%). If instead we regard the aptamer sequences as selected against LXRβ, the cross-reactivities decrease considerably, to 17% for LXRα and 7% for RXRα. Therefore, in the future we are planning to use the obtained aptamers as binders for LXRβ.

Original languageEnglish (US)
Pages (from-to)512-517
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume332
Issue number2
DOIs
StatePublished - Jul 1 2005

Keywords

  • Aptamer
  • LXRα
  • LXRβ
  • SELEX
  • SPR

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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