Seasonal H3N2 influenza A virus fails to enhance Staphylococcus aureus co-infection in a non-human primate respiratory tract infection model

Scott D. Kobayashi, Randall J. Olsen, Rachel A. LaCasse, David Safronetz, Madiha Ashraf, Adeline R. Porter, Kevin R. Braughton, Friederike Feldmann, Dawn R. Clifton, John C. Kash, John R. Bailey, Donald J. Gardner, Michael Otto, Douglas L. Brining, Barry N. Kreiswirth, Jeffrey K. Taubenberger, Michael J. Parnell, Heinz Feldmann, James M. Musser, Frank R. DeLeo

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Staphylococcus aureus community-acquired pneumonia is often associated with influenza or an influenza-like syndrome. Morbidity and mortality due to methicillin-resistant S. aureus (MRSA) or influenza and pneumonia, which includes bacterial co-infection, are among the top causes of death by infectious diseases in the United States. We developed a non-lethal influenza A virus (IAV) (H3N2)/S. aureus co-infection model in cynomolgus macaques (Macaca fascicularis) to test the hypothesis that seasonal IAV infection predisposes non-human primates to severe S. aureus pneumonia. Infection and disease progression were monitored by clinical assessment of animal health; analysis of blood chemistry, nasal swabs, and X-rays; and gross pathology and histopathology of lungs from infected animals. Seasonal IAV infection in healthy cynomolgus macaques caused mild pneumonia, but unexpectedly, did not predispose these animals to subsequent severe infection with the community-associated MRSA clone USA300. We conclude that in our co-infection model, seasonal IAV infection alone is not sufficient to promote severe S. aureus pneumonia in otherwise healthy non-human primates. The implication of these findings is that comorbidity factors in addition to IAV infection are required to predispose individuals to secondary S. aureus pneumonia.

Original languageEnglish (US)
JournalVirulence
Volume4
Issue number8
DOIs
StatePublished - 2013

Keywords

  • Coinfection
  • Influenza a virus
  • MRSA
  • Pneumonia
  • Staphylococcus aureus
  • USA300

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

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