TY - JOUR
T1 - Sealing of polyester prostheses with autologous fibrin glue and bone marrow
AU - Cardon, Alain
AU - Chakfé, Nabil
AU - Thaveau, Fabien
AU - Gagnon, Edith
AU - Hartung, Olivier
AU - Aillet, Sylvie
AU - Kerdiles, Yvon
AU - Dion, Yves Marie
AU - Kretz, Jean Georges
AU - Doillon, Charles J.
N1 - Funding Information:
This study was supported by grants from the Fondation de I'Avenir pour la Recherche Mbdicale Appliqu& and the Socibtb de Chirurgie Vasculaire de Langue Frangaise. We are grateful to Mr. Aristide Pusterla and Mr. Richard Janvier for technical assistance, Dr. Jim Gourdon and his co-workers for veterinarian expertise, and the Cardial SA Company for donating the prosthetic grafts.
PY - 2000
Y1 - 2000
N2 - The purpose of this study was to develop a sealing technique for polyester prosthetic grafts able to promote healing and reduce intimal hyperplasia. The porcine experimental model was aortoiliac bypass with a 6-mm diameter knitted polyester prosthetic graft implanted for 14 and 90 days. Animals were divided into three groups according to sealing technique as follows: preclotting with blood (group I, n = 12), sealing with autologous fibrin glue (group II, n = 14), and sealing with autologous fibrin glue and bone marrow cells (group III, n = 16). Feasibility and quality of sealing were evaluated by scanning electron microscopy prior to implantation and by assessment of blood loss. After removal, prostheses were cut into three segments comprising the proximal anastomosis, midsection, and distal anastomosis. Pieces were fixed, embedded in paraffin, and serially sectioned for histologic study. Histological study focused on the degree of stenosis and hyperplasia of the neointima of each prosthesis. The results of this short-term study indicate that sealing of polyester vascular prosthetic grafts with autologous fibrin glue and bone marrow cells is effective in reducing intimal hyperplasia. However further study will be needed to assess long-term healing.
AB - The purpose of this study was to develop a sealing technique for polyester prosthetic grafts able to promote healing and reduce intimal hyperplasia. The porcine experimental model was aortoiliac bypass with a 6-mm diameter knitted polyester prosthetic graft implanted for 14 and 90 days. Animals were divided into three groups according to sealing technique as follows: preclotting with blood (group I, n = 12), sealing with autologous fibrin glue (group II, n = 14), and sealing with autologous fibrin glue and bone marrow cells (group III, n = 16). Feasibility and quality of sealing were evaluated by scanning electron microscopy prior to implantation and by assessment of blood loss. After removal, prostheses were cut into three segments comprising the proximal anastomosis, midsection, and distal anastomosis. Pieces were fixed, embedded in paraffin, and serially sectioned for histologic study. Histological study focused on the degree of stenosis and hyperplasia of the neointima of each prosthesis. The results of this short-term study indicate that sealing of polyester vascular prosthetic grafts with autologous fibrin glue and bone marrow cells is effective in reducing intimal hyperplasia. However further study will be needed to assess long-term healing.
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U2 - 10.1007/s100169910102
DO - 10.1007/s100169910102
M3 - Article
C2 - 11128447
AN - SCOPUS:0033657849
SN - 0890-5096
VL - 14
SP - 543
EP - 552
JO - Annals of Vascular Surgery
JF - Annals of Vascular Surgery
IS - 6
ER -