SDH2is involved in proper hypha formation and virulence in Candida albicans

Shuang Bi; Quan Zhen Lv; Tian Tian Wang; Beth Burgwyn Fuchs; Dan Dan Hu; Cleo G. Anastassopoulou; Athanasios Desalermos; Maged Muhammed; Chin Lee Wu; Yuan Ying Jiang; Eleftherios Mylonakis; Yan Wang

doi:10.2217/fmb-2018-0033

SDH2is involved in proper hypha formation and virulence in Candida albicans

Shuang Bi, Quan Zhen Lv, Tian Tian Wang, Beth Burgwyn Fuchs, Dan Dan Hu, Cleo G. Anastassopoulou, Athanasios Desalermos, Maged Muhammed, Chin Lee Wu, Yuan Ying Jiang, Eleftherios Mylonakis, Yan Wang

Houston Methodist

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Aim: To investigate the role of SDH2 in Candida albicans filamentation and virulence. Materials & methods: Caenorhabditis elegans and mouse candidiasis models were used to assess the virulence of a sdh2Δ/Δ mutant. Various hypha-inducing media were used to evaluate the hyphal development of C. albicans. DCFH-DA was used to measure intracellular Reactive Oxygen Species (ROS) levels. Results: The sdh2Δ/Δ mutant was avirulent in the C. elegans model, hypovirulent in a murine candidiasis model, and defective to form filaments both in vitro and in vivo. Intracellular ROS level increased in the sdh2Δ/Δ mutant, and the filamentation defects of sdh2Δ/Δ were rescued by decreasing intracellular ROS. Conclusion: SDH2 plays an important role in C. albicans filamentation and virulence probably through affecting intracellular ROS. </inline-graphic.

Original language	English (US)
Pages (from-to)	1141-1155
Number of pages	15
Journal	Future Microbiology
Volume	13
Issue number	10
DOIs	https://doi.org/10.2217/fmb-2018-0033
State	Published - Aug 2018

Keywords

Candida albicans
morphogenesis
succinate dehydrogenase
virulence

ASJC Scopus subject areas

Microbiology
Microbiology (medical)

Divisions

Infectious Disease

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10.2217/fmb-2018-0033

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@article{dd8e1361399f43e981492b96aa60cc7a,

title = "SDH2is involved in proper hypha formation and virulence in Candida albicans",

abstract = "Aim: To investigate the role of SDH2 in Candida albicans filamentation and virulence. Materials \& methods: Caenorhabditis elegans and mouse candidiasis models were used to assess the virulence of a sdh2Δ/Δ mutant. Various hypha-inducing media were used to evaluate the hyphal development of C. albicans. DCFH-DA was used to measure intracellular Reactive Oxygen Species (ROS) levels. Results: The sdh2Δ/Δ mutant was avirulent in the C. elegans model, hypovirulent in a murine candidiasis model, and defective to form filaments both in vitro and in vivo. Intracellular ROS level increased in the sdh2Δ/Δ mutant, and the filamentation defects of sdh2Δ/Δ were rescued by decreasing intracellular ROS. Conclusion: SDH2 plays an important role in C. albicans filamentation and virulence probably through affecting intracellular ROS. ",

keywords = "Candida albicans, morphogenesis, succinate dehydrogenase, virulence",

author = "Shuang Bi and Lv, \{Quan Zhen\} and Wang, \{Tian Tian\} and Fuchs, \{Beth Burgwyn\} and Hu, \{Dan Dan\} and Anastassopoulou, \{Cleo G.\} and Athanasios Desalermos and Maged Muhammed and Wu, \{Chin Lee\} and Jiang, \{Yuan Ying\} and Eleftherios Mylonakis and Yan Wang",

note = "Publisher Copyright: {\textcopyright} 2018 2018 Future Medicine Ltd.",

year = "2018",

month = aug,

doi = "10.2217/fmb-2018-0033",

language = "English (US)",

volume = "13",

pages = "1141--1155",

journal = "Future Microbiology",

issn = "1746-0913",

publisher = "Taylor and Francis Ltd.",

number = "10",

}

TY - JOUR

T1 - SDH2is involved in proper hypha formation and virulence in Candida albicans

AU - Bi, Shuang

AU - Lv, Quan Zhen

AU - Wang, Tian Tian

AU - Fuchs, Beth Burgwyn

AU - Hu, Dan Dan

AU - Anastassopoulou, Cleo G.

AU - Desalermos, Athanasios

AU - Muhammed, Maged

AU - Wu, Chin Lee

AU - Jiang, Yuan Ying

AU - Mylonakis, Eleftherios

AU - Wang, Yan

PY - 2018/8

Y1 - 2018/8

N2 - Aim: To investigate the role of SDH2 in Candida albicans filamentation and virulence. Materials & methods: Caenorhabditis elegans and mouse candidiasis models were used to assess the virulence of a sdh2Δ/Δ mutant. Various hypha-inducing media were used to evaluate the hyphal development of C. albicans. DCFH-DA was used to measure intracellular Reactive Oxygen Species (ROS) levels. Results: The sdh2Δ/Δ mutant was avirulent in the C. elegans model, hypovirulent in a murine candidiasis model, and defective to form filaments both in vitro and in vivo. Intracellular ROS level increased in the sdh2Δ/Δ mutant, and the filamentation defects of sdh2Δ/Δ were rescued by decreasing intracellular ROS. Conclusion: SDH2 plays an important role in C. albicans filamentation and virulence probably through affecting intracellular ROS.

AB - Aim: To investigate the role of SDH2 in Candida albicans filamentation and virulence. Materials & methods: Caenorhabditis elegans and mouse candidiasis models were used to assess the virulence of a sdh2Δ/Δ mutant. Various hypha-inducing media were used to evaluate the hyphal development of C. albicans. DCFH-DA was used to measure intracellular Reactive Oxygen Species (ROS) levels. Results: The sdh2Δ/Δ mutant was avirulent in the C. elegans model, hypovirulent in a murine candidiasis model, and defective to form filaments both in vitro and in vivo. Intracellular ROS level increased in the sdh2Δ/Δ mutant, and the filamentation defects of sdh2Δ/Δ were rescued by decreasing intracellular ROS. Conclusion: SDH2 plays an important role in C. albicans filamentation and virulence probably through affecting intracellular ROS.

KW - Candida albicans

KW - morphogenesis

KW - succinate dehydrogenase

KW - virulence

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U2 - 10.2217/fmb-2018-0033

DO - 10.2217/fmb-2018-0033

M3 - Article

C2 - 30113213

AN - SCOPUS:85053249961

SN - 1746-0913

VL - 13

SP - 1141

EP - 1155

JO - Future Microbiology

JF - Future Microbiology

IS - 10

ER -

SDH2is involved in proper hypha formation and virulence in Candida albicans

Abstract

Keywords

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