SDH2is involved in proper hypha formation and virulence in Candida albicans

Shuang Bi; Quan Zhen Lv; Tian Tian Wang; Beth Burgwyn Fuchs; Dan Dan Hu; Cleo G. Anastassopoulou; Athanasios Desalermos; Maged Muhammed; Chin Lee Wu; Yuan Ying Jiang; Eleftherios Mylonakis; Yan Wang

doi:10.2217/fmb-2018-0033

SDH2is involved in proper hypha formation and virulence in Candida albicans

Shuang Bi, Quan Zhen Lv, Tian Tian Wang, Beth Burgwyn Fuchs, Dan Dan Hu, Cleo G. Anastassopoulou, Athanasios Desalermos, Maged Muhammed, Chin Lee Wu, Yuan Ying Jiang, Eleftherios Mylonakis, Yan Wang

Houston Methodist

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Aim: To investigate the role of SDH2 in Candida albicans filamentation and virulence. Materials & methods: Caenorhabditis elegans and mouse candidiasis models were used to assess the virulence of a sdh2Δ/Δ mutant. Various hypha-inducing media were used to evaluate the hyphal development of C. albicans. DCFH-DA was used to measure intracellular Reactive Oxygen Species (ROS) levels. Results: The sdh2Δ/Δ mutant was avirulent in the C. elegans model, hypovirulent in a murine candidiasis model, and defective to form filaments both in vitro and in vivo. Intracellular ROS level increased in the sdh2Δ/Δ mutant, and the filamentation defects of sdh2Δ/Δ were rescued by decreasing intracellular ROS. Conclusion: SDH2 plays an important role in C. albicans filamentation and virulence probably through affecting intracellular ROS. </inline-graphic.

Original language	English (US)
Pages (from-to)	1141-1155
Number of pages	15
Journal	Future Microbiology
Volume	13
Issue number	10
DOIs	https://doi.org/10.2217/fmb-2018-0033
State	Published - Aug 2018

Keywords

Candida albicans
morphogenesis
succinate dehydrogenase
virulence

ASJC Scopus subject areas

Microbiology
Microbiology (medical)

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10.2217/fmb-2018-0033

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@article{dd8e1361399f43e981492b96aa60cc7a,

title = "SDH2is involved in proper hypha formation and virulence in Candida albicans",

abstract = "Aim: To investigate the role of SDH2 in Candida albicans filamentation and virulence. Materials & methods: Caenorhabditis elegans and mouse candidiasis models were used to assess the virulence of a sdh2Δ/Δ mutant. Various hypha-inducing media were used to evaluate the hyphal development of C. albicans. DCFH-DA was used to measure intracellular Reactive Oxygen Species (ROS) levels. Results: The sdh2Δ/Δ mutant was avirulent in the C. elegans model, hypovirulent in a murine candidiasis model, and defective to form filaments both in vitro and in vivo. Intracellular ROS level increased in the sdh2Δ/Δ mutant, and the filamentation defects of sdh2Δ/Δ were rescued by decreasing intracellular ROS. Conclusion: SDH2 plays an important role in C. albicans filamentation and virulence probably through affecting intracellular ROS. ",

keywords = "Candida albicans, morphogenesis, succinate dehydrogenase, virulence",

author = "Shuang Bi and Lv, {Quan Zhen} and Wang, {Tian Tian} and Fuchs, {Beth Burgwyn} and Hu, {Dan Dan} and Anastassopoulou, {Cleo G.} and Athanasios Desalermos and Maged Muhammed and Wu, {Chin Lee} and Jiang, {Yuan Ying} and Eleftherios Mylonakis and Yan Wang",

note = "Funding Information: This work was supported by the National Natural Science Foundation of China (81273558 and 81772124), and the NIH through an R01 award (AI075286), and an R21 award (AI070569). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript. Publisher Copyright: {\textcopyright} 2018 2018 Future Medicine Ltd.",

year = "2018",

month = aug,

doi = "10.2217/fmb-2018-0033",

language = "English (US)",

volume = "13",

pages = "1141--1155",

journal = "Future Microbiology",

issn = "1746-0913",

publisher = "Future Medicine Ltd.",

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TY - JOUR

T1 - SDH2is involved in proper hypha formation and virulence in Candida albicans

AU - Bi, Shuang

AU - Lv, Quan Zhen

AU - Wang, Tian Tian

AU - Fuchs, Beth Burgwyn

AU - Hu, Dan Dan

AU - Anastassopoulou, Cleo G.

AU - Desalermos, Athanasios

AU - Muhammed, Maged

AU - Wu, Chin Lee

AU - Jiang, Yuan Ying

AU - Mylonakis, Eleftherios

AU - Wang, Yan

N1 - Funding Information: This work was supported by the National Natural Science Foundation of China (81273558 and 81772124), and the NIH through an R01 award (AI075286), and an R21 award (AI070569). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript. Publisher Copyright: © 2018 2018 Future Medicine Ltd.

PY - 2018/8

Y1 - 2018/8

N2 - Aim: To investigate the role of SDH2 in Candida albicans filamentation and virulence. Materials & methods: Caenorhabditis elegans and mouse candidiasis models were used to assess the virulence of a sdh2Δ/Δ mutant. Various hypha-inducing media were used to evaluate the hyphal development of C. albicans. DCFH-DA was used to measure intracellular Reactive Oxygen Species (ROS) levels. Results: The sdh2Δ/Δ mutant was avirulent in the C. elegans model, hypovirulent in a murine candidiasis model, and defective to form filaments both in vitro and in vivo. Intracellular ROS level increased in the sdh2Δ/Δ mutant, and the filamentation defects of sdh2Δ/Δ were rescued by decreasing intracellular ROS. Conclusion: SDH2 plays an important role in C. albicans filamentation and virulence probably through affecting intracellular ROS.

AB - Aim: To investigate the role of SDH2 in Candida albicans filamentation and virulence. Materials & methods: Caenorhabditis elegans and mouse candidiasis models were used to assess the virulence of a sdh2Δ/Δ mutant. Various hypha-inducing media were used to evaluate the hyphal development of C. albicans. DCFH-DA was used to measure intracellular Reactive Oxygen Species (ROS) levels. Results: The sdh2Δ/Δ mutant was avirulent in the C. elegans model, hypovirulent in a murine candidiasis model, and defective to form filaments both in vitro and in vivo. Intracellular ROS level increased in the sdh2Δ/Δ mutant, and the filamentation defects of sdh2Δ/Δ were rescued by decreasing intracellular ROS. Conclusion: SDH2 plays an important role in C. albicans filamentation and virulence probably through affecting intracellular ROS.

KW - Candida albicans

KW - morphogenesis

KW - succinate dehydrogenase

KW - virulence

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SN - 1746-0913

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ER -

SDH2is involved in proper hypha formation and virulence in Candida albicans

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Keywords

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