Abstract
Aim: To investigate the role of SDH2 in Candida albicans filamentation and virulence. Materials & methods: Caenorhabditis elegans and mouse candidiasis models were used to assess the virulence of a sdh2Δ/Δ mutant. Various hypha-inducing media were used to evaluate the hyphal development of C. albicans. DCFH-DA was used to measure intracellular Reactive Oxygen Species (ROS) levels. Results: The sdh2Δ/Δ mutant was avirulent in the C. elegans model, hypovirulent in a murine candidiasis model, and defective to form filaments both in vitro and in vivo. Intracellular ROS level increased in the sdh2Δ/Δ mutant, and the filamentation defects of sdh2Δ/Δ were rescued by decreasing intracellular ROS. Conclusion: SDH2 plays an important role in C. albicans filamentation and virulence probably through affecting intracellular ROS. </inline-graphic.
Original language | English (US) |
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Pages (from-to) | 1141-1155 |
Number of pages | 15 |
Journal | Future Microbiology |
Volume | 13 |
Issue number | 10 |
DOIs | |
State | Published - Aug 2018 |
Keywords
- Candida albicans
- morphogenesis
- succinate dehydrogenase
- virulence
ASJC Scopus subject areas
- Microbiology
- Microbiology (medical)