Screening protocol for Torulopsis (Candida) glabrata

Geoffrey Land, Jackie Burke, Carol Shelby, Judith Rhodes, Joyce Collett, Isabelle Bennett, Jettie Johnson

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

A screening test has been developed for the presumptive identification of Torulopsis (Candida) glabrata from other common clinical isolates of yeast-like fungi. An interlaboratory comparison of a protocol consisting of morphology on cornmeal Tween 80 agar and trehalose fermentation at 42°C was successful in differentiating T. glabrata from other taxa that are frequent or possible clinical isolates. The screening results for 517 clinical yeast isolates, 241 of which were T. glabrata, were compared with their final identification via commercial systems (API 20C Yeast Identification System [bioMERIEUX, Hazelwood, Mo.] and Rapid Yeast Identification Panel [Dade Microscan, Sacramento, Calif.]). The trehalose screening test has a sensitivity and a specificity of 97.8 and 95.8%, respectively, and a positive predictive value of 97.4% and a negative predictive value of 96.5%. Overall, the trehalose screen had an efficiency rating of 93.9% for ruling in or out T. glabrata. Since T. glabrata represents a substantial part of the workload in a clinical laboratory, a significant reduction in direct and indirect costs should be realized.

Original languageEnglish (US)
Pages (from-to)2300-2303
Number of pages4
JournalJournal of Clinical Microbiology
Volume34
Issue number9
DOIs
StatePublished - Sep 1996
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)

Fingerprint Dive into the research topics of 'Screening protocol for Torulopsis (Candida) glabrata'. Together they form a unique fingerprint.

Cite this