TY - JOUR
T1 - Screening for genes preferentially expressed in the early phase of chondrogenesis
AU - Shinomura, Tamayuki
AU - Ito, Kazuo
AU - Kimura, James H.
AU - Höök, Magnus
N1 - Funding Information:
We are grateful to Dr. Takashi Yokochi (Department of Microbiology and Immunology, Aichi Medical University) for his continuous encouragements. This work was supported by Grants-in-Aid for Scientific Research (13470305 and 14657360) from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 2006/3/3
Y1 - 2006/3/3
N2 - This study reports a new system that is very effective in identifying genes closely related to the early phase of chondrogenic differentiation. While studying chondrogenesis in a progenitor cell line, ATDC5, we found that the amount of culture media overlying an ATDC5 monolayer affected the extent to which differentiation occurred. Therefore, to gain insight into the molecular mechanisms of chondrogenic differentiation, differential gene expression between differentiating and non-differentiating ATDC5 cultures was examined by suppression subtractive hybridization analysis. In this study, we focused on transcription factors that were identified in differentiating cultures, and found that activating transcription factor 5, ATF5, exhibited a conspicuous activation pattern using two methods to induce chondrogenesis of ATDC5 cells. Furthermore, ATF5 was found to be elevated in the developing limb bud by in situ hybridization in a pattern that was highly restricted to the cartilaginous anlagen, suggesting a positive association with ATF5 expression and chondrogenesis.
AB - This study reports a new system that is very effective in identifying genes closely related to the early phase of chondrogenic differentiation. While studying chondrogenesis in a progenitor cell line, ATDC5, we found that the amount of culture media overlying an ATDC5 monolayer affected the extent to which differentiation occurred. Therefore, to gain insight into the molecular mechanisms of chondrogenic differentiation, differential gene expression between differentiating and non-differentiating ATDC5 cultures was examined by suppression subtractive hybridization analysis. In this study, we focused on transcription factors that were identified in differentiating cultures, and found that activating transcription factor 5, ATF5, exhibited a conspicuous activation pattern using two methods to induce chondrogenesis of ATDC5 cells. Furthermore, ATF5 was found to be elevated in the developing limb bud by in situ hybridization in a pattern that was highly restricted to the cartilaginous anlagen, suggesting a positive association with ATF5 expression and chondrogenesis.
KW - Activating transcription factor 5
KW - ATDC5 cells
KW - Chondrogenesis
KW - Glucocorticoid-induced leucine zipper
KW - Inclined culture
KW - Pleiotrophin
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U2 - 10.1016/j.bbrc.2005.12.169
DO - 10.1016/j.bbrc.2005.12.169
M3 - Article
C2 - 16412979
AN - SCOPUS:30944457984
VL - 341
SP - 167
EP - 174
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -