Screening far red probes for use on optical biochip devices

Kerenza L. Njoh, Laurence H. Patterson, Klaus Pors, Mire Zloh, Simon Ameer-Beg, Huw Summers, Daniel Matthews, Rachel J. Errington, Paul J. Smith

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In situ spectral analysis can be used to understand the targeting and interaction of agents in cellular compartments. A range of novel red excitable fluorescent probes, related to the anthraquinone family of anti-cancer agents, were designed for their DNA affinic properties and their ability to enter and penetrate living cells. We report on the spectral features of these probes, both in solution and bound within intact cells, to identify unique fluorescent signatures that exploit their use in bioassays on optical biochip devices. The probes demonstrated red shifted emission spectra and increased 2 photon lifetime, with minimal fluorescent enhancement, upon binding to DNA. Spectral confocal laser scanning microscopy revealed complex emission profiles representing the bound (nuclear) and unbound (cytoplasmic) fractions of the DNA probes within live interphase, mitotic and apoptotic cells. Analysis of the emission peaks encoded the spectra to provide cell compartment recognition and profiles for cells in different cell states. Sampling the entire emission spectra of these probes for cell locating, even in the presence of unbound molecules, provides good signal-to-noise in biochip devices. Furthermore, by sampling the fluorescence output at specific spectral windows we can obtain high spatial information without imaging. The technological challenge is to integrate these fluorophores and appropriate detection capacity onto an optical biochip platform with microfluidic systems for cell handling.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
DOIs
StatePublished - 2006
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV - San Jose, CA, United States
Duration: Jan 23 2006Jan 25 2006

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6088
ISSN (Print)1605-7422

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV
CountryUnited States
CitySan Jose, CA
Period1/23/061/25/06

Keywords

  • Anthraquinone
  • DNA intercalating
  • Fluorescence spectral imaging
  • Optical biochips

ASJC Scopus subject areas

  • Engineering(all)

Fingerprint Dive into the research topics of 'Screening far red probes for use on optical biochip devices'. Together they form a unique fingerprint.

Cite this