Screening and identification of host factors interacting with UL14 of herpes simplex virus 1

Fuqing Wu, Junji Xing, Shuai Wang, Meili Li, Chunfu Zheng

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The UL14 protein of herpes simplex virus type 1 (HSV-1) is highly conserved in herpesvirus family. However, its exact function during the HSV-1 replication cycle is little known. In the present study, a high throughput yeast two-hybrid system was employed to screen the cellular factors interacting with UL14, and five target candidates were yielded: (1) TSC22 domain family protein 3 (TSC22D3); (2) Mediator of RNA polymerase II transcription subunit 8 isoform 1(MED8); (3) Runt-related transcription factor 3 (RUNX3); (4) Arrestin beta-2 (ARRB2); (5) Cereblon (CRBN). Indirect immunofluorescent assay showed that both TSC22D3 and MED8 co-localized with UL14. Co-immunoprecipitation assay demonstrated that UL14 could be immunoprecipitated by TSC22D3, suggesting that UL14 interacted with TSC22D3 under physiological condition. In summary, this study opened up new avenues toward delineating the function and physiological significance of UL14 during the HSV-1 replication cycle.

Original languageEnglish (US)
Pages (from-to)203-208
Number of pages6
JournalMedical Microbiology and Immunology
Volume200
Issue number3
DOIs
StatePublished - Aug 2011

Keywords

  • Co-immunoprecipitation
  • Co-localization
  • Herpes simplex virus 1
  • UL14
  • Yeast two-hybrid

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Microbiology (medical)

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