Roles of E-cadherin in enhancing the in vitro differentiation of hepatocytes from murine embryonic stem ceils

An Bin Hu, Chang Zhen Shang, Jun Min, Ji Ye Cai, Xiaoshun He

Research output: Contribution to journalArticle

Abstract

Objective: To investigate the roles of E-cadherin (E-cad) in enhancing the in vitro differentiation of hepatocytes from murine embryonic stem cells (ESCs). Methods: Exogenous E-cad was transfected into BALB/c murine ESCs to enable its stable expression. Then hepatic differentiation from E-cad-ESCs was induced by such growth factors as hepatocyte growth factor (HGF), fibroblast growth factor (FGE) and transforming growth factor (TGF). And the expressions of hepatic markers ALB, TAT, Cyp7a1 and urea were detected. The morphology of hepatic differentiation was observed under microscopy. Results: E-cad expression gradually decreased in normal ESC differentiation, but was stably expressed in E-cad-ESCs. In E-cad-ESC group, hepatic markers ALB, TAT and CYP7a1 were expressed earlier or higher than that in normal ESC group, and the concentrations of ALB and urea were significantly higher than that in normal ESC group. The adhesion of the differentiated E-cad-ESCs was significantly enhanced compared with the normal ESCs. They maintained close connections and multidimensional growth. Cell number of hepatocytes from ESC increased significantly in E-cad-ESC group. Conclusion: E-cad enhances the hepatic differentiation of ESC by increasing the number of differentiated cells and increasing the synthetic capacity of ALB and urea.

Original languageEnglish (US)
Pages (from-to)3143-3146
Number of pages4
JournalNational Medical Journal of China
Volume92
Issue number44
DOIs
StatePublished - Nov 27 2012

Keywords

  • Cell differentiation
  • Embryonic stem cells
  • Epithelial cadherin
  • Hepatocytes

ASJC Scopus subject areas

  • Medicine(all)

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