DNA molecules of three sizes (411 bp, 48.5 kbp, and 167 kbp) were analyzed in an asymmetric obstacle array. Array boundary conditions of carefully aligned current were used to create very straight bands over long distances, and the separation of molecules was studied as a function of drift velocity. Evidence was obtained that fractionation in asymmetric obstacle arrays requires that the molecular size be comparable with the barrier gap size.
ASJC Scopus subject areas
- Physics and Astronomy(all)