Role of molecular size in ratchet fractionation

Lotien Richard Huang; Pascal Silberzan; Jonas O. Tegenfeldt; Edward C. Cox; James C. Sturm; R. Austin; Harold Craighead

doi:10.1103/PhysRevLett.89.178301

Role of molecular size in ratchet fractionation

Lotien Richard Huang, Pascal Silberzan, Jonas O. Tegenfeldt, Edward C. Cox, James C. Sturm, R. Austin, Harold Craighead

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73 Scopus citations

Abstract

DNA molecules of three sizes (411 bp, 48.5 kbp, and 167 kbp) were analyzed in an asymmetric obstacle array. Array boundary conditions of carefully aligned current were used to create very straight bands over long distances, and the separation of molecules was studied as a function of drift velocity. Evidence was obtained that fractionation in asymmetric obstacle arrays requires that the molecular size be comparable with the barrier gap size.

Original language	English (US)
Article number	178301
Pages (from-to)	178301/1-178301/4
Journal	Physical Review Letters
Volume	89
Issue number	17
DOIs	https://doi.org/10.1103/PhysRevLett.89.178301
State	Published - Oct 21 2002

ASJC Scopus subject areas

Physics and Astronomy(all)

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10.1103/PhysRevLett.89.178301

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title = "Role of molecular size in ratchet fractionation",

abstract = "DNA molecules of three sizes (411 bp, 48.5 kbp, and 167 kbp) were analyzed in an asymmetric obstacle array. Array boundary conditions of carefully aligned current were used to create very straight bands over long distances, and the separation of molecules was studied as a function of drift velocity. Evidence was obtained that fractionation in asymmetric obstacle arrays requires that the molecular size be comparable with the barrier gap size.",

author = "Huang, {Lotien Richard} and Pascal Silberzan and Tegenfeldt, {Jonas O.} and Cox, {Edward C.} and Sturm, {James C.} and R. Austin and Harold Craighead",

note = "Funding Information: This work was supported by grants from DARPA (MDA972-00-1-0031), NIH (HG01506), and the State of New Jersey (NJCST 99-100-082-2042-007). P. Silberzan gratefully thanks the DGA for financial ?>support.",

year = "2002",

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doi = "10.1103/PhysRevLett.89.178301",

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T1 - Role of molecular size in ratchet fractionation

AU - Huang, Lotien Richard

AU - Silberzan, Pascal

AU - Tegenfeldt, Jonas O.

AU - Cox, Edward C.

AU - Sturm, James C.

AU - Austin, R.

AU - Craighead, Harold

N1 - Funding Information: This work was supported by grants from DARPA (MDA972-00-1-0031), NIH (HG01506), and the State of New Jersey (NJCST 99-100-082-2042-007). P. Silberzan gratefully thanks the DGA for financial ?>support.

PY - 2002/10/21

Y1 - 2002/10/21

N2 - DNA molecules of three sizes (411 bp, 48.5 kbp, and 167 kbp) were analyzed in an asymmetric obstacle array. Array boundary conditions of carefully aligned current were used to create very straight bands over long distances, and the separation of molecules was studied as a function of drift velocity. Evidence was obtained that fractionation in asymmetric obstacle arrays requires that the molecular size be comparable with the barrier gap size.

AB - DNA molecules of three sizes (411 bp, 48.5 kbp, and 167 kbp) were analyzed in an asymmetric obstacle array. Array boundary conditions of carefully aligned current were used to create very straight bands over long distances, and the separation of molecules was studied as a function of drift velocity. Evidence was obtained that fractionation in asymmetric obstacle arrays requires that the molecular size be comparable with the barrier gap size.

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JO - Physical Review Letters

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Role of molecular size in ratchet fractionation

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