@article{89024045b1cd435fab87f7930b698d3b,
title = "Role of c-Jun terminal kinase (JNK) activation in influenza A virus-induced autophagy and replication",
abstract = "The non-structural protein 1 (NS1) of different influenza A virus (IAV) strains can differentially regulate the activity of c-Jun terminal kinase (JNK) and PI-3 kinase (PI3K). Whether varying JNK and PI3K activation impacts autophagy and IAV replication differently remains uncertain. Here we report that H5N1 (A/mallard/Huadong/S/2005) influenza A virus induced functional autophagy, as evidenced by increased LC3 lipidation and decreased p62 levels, and the presence of autolysosomes in chicken fibroblast cells. H9N2 (A/chicken/Shanghai/F/98) virus weakly induced autophagy, whereas H1N1 virus (A/PR/8/34, PR8) blocked autophagic flux. H5N1 virus activated JNK but inhibited the PI-3 kinase pathway. In contrast, N9N2 virus infection led to modest JNK activation and strong PI-3 kinase activation; whereas H1N1 virus activated the PI-3 kinase pathway but did not activate JNK. SP600125, a JNK inhibitor, inhibited H5N1 virus-induced autophagy and virus replication in a DF-1 chicken fibroblast cell line. Our study uncovered a previously unrecognized role of JNK in IAV replication and autophagy.",
keywords = "Autophagy, Influenza A virus, JNK, NS1, PI-3 kinase",
author = "Jingting Zhang and Tao Ruan and Tianyu Sheng and Jiongjiong Wang and Jing Sun and Jin Wang and Prinz, {Richard A.} and Daxin Peng and Xiufan Liu and Xiulong Xu",
note = "Funding Information: This work was supported in part by a start-up fund from the College of Veterinary Medicine, Yangzhou University, and the Priority Academic Program Development of Jiangsu Higher Education Institutions to Xiulong Xu, and by China Postdoctoral Science Foundation (2015M581873), Natural Science Foundation of Jiangsu Province (BK20150450), and the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry (2015311) to Jing Sun. We are very grateful to Dr. Liqian Zhu for kindly providing H1N1 virus. Funding Information: This work was supported in part by a start-up fund from the College of Veterinary Medicine, Yangzhou University , and the Priority Academic Program Development of Jiangsu Higher Education Institutions to Xiulong Xu, and by China Postdoctoral Science Foundation ( 2015M581873 ), Natural Science Foundation of Jiangsu Province ( BK20150450 ), and the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry ( 2015311 ) to Jing Sun. We are very grateful to Dr. Liqian Zhu for kindly providing H1N1 virus. Publisher Copyright: {\textcopyright} 2018 Elsevier Inc. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.",
year = "2019",
month = jan,
day = "2",
doi = "10.1016/j.virol.2018.09.020",
language = "English (US)",
volume = "526",
pages = "1--12",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press",
}